The impact of ovarian stimulation on implantation and fetal development in mice
The impact of ovarian stimulation on implantation and fetal development in mice
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DOI：
10.1093/humrep/16.2.221
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发表时间：
2001-02-01
影响因子：
6.1
通讯作者：
Storeng, R
中科院分区：
文献类型：
--
作者：
Ertzeid, G;Storeng, R
本研究的目的是评估，使用胚胎捐赠模型，是否受损的卵母细胞/胚胎发育能力和/或子宫环境的变化是负责先前观察到的不良影响与促性腺激素超排卵的植入和胎儿发育的小鼠。将来自超排卵和非刺激雌性的胚胎转移到相同超排卵或非刺激假孕受体小鼠内的单独子宫角。胚胎发育受损，因为与超数排卵供体（41%; P = 0.001）相比，对照供体（61%）的正常胚胎在移植当天为囊胚。移植超数排卵供体胚胎后，对照受体的着床率（12%）显著低于对照供体（25%; P = 0.001）。超数排卵受者子宫容受性受损。对照组胚胎的种植率（25%）显著高于超数排卵受体（7%; P = 0.001）。移植从超排供体回收的胚胎导致超排受体的植入后胎儿死亡率（69%）显著高于对照受体（36%; P = 0.01），并且从超数排卵受体获得的胎儿的平均活胎重量显著较低（0.51 g）与从对照受体获得的胎儿（0.72 g; P = 0.006）相比。因此，卵巢刺激似乎损害卵母细胞/胚胎质量以及子宫环境。
The objective of this study was to evaluate, using an embryo donation model, whether impaired oocyte/embryo developmental competence and/or changes in uterine milieu are responsible for the previously observed adverse effects of superovulation with gonadotrophins on implantation and fetal development in mice. Embryos from superovulated and non-stimulated females were transferred to separate uterine horns within the same superovulated or non-stimulated pseudopregnant recipient mice. Embryo development was impaired as a significantly higher proportion of normal embryos from control donors (61%) were blastocysts on transfer day compared with superovulated donors (41%; P = 0.001). The implantation rate in control recipients was significantly reduced after transfer of embryos from superovulated donors (12%) compared with control donors (25%; P = 0.001). Uterine receptivity was impaired in superovulated recipients. The implantation rate of control embryos was significantly higher in control (25%) than in superovulated recipients (7%; P = 0.001). Transfer of embryos recovered from snperovulated donors resulted in significantly higher post-implantation fetal mortality in superovulated recipients (69%) than in control recipients (36%; P = 0.01), and the mean weight of live fetuses was significantly lower for fetuses obtained from superovulated recipients (0.51 g) compared with that of fetuses obtained from control recipients (0.72 g; P = 0.006). Hence, ovarian stimulation appears to impair oocyte/embryo quality as well as uterine milieu.