Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions
Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions
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DOI：
10.1016/s0142-9612(03)00117-0
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发表时间：
2003-08-01
期刊：
影响因子：
14
通讯作者：
Zhang, XF
中科院分区：
文献类型：
--
作者：
Cheng, MY;Deng, JU;Zhang, XF
通过改变组分的配比，制备了一系列壳聚糖-明胶复合薄膜。FT-IR和x射线分析表明这两种生物聚合物具有良好的相容性。差示扫描量热法（DSC）分析表明，与明胶混合后，壳聚糖膜的吸水率增加。与壳聚糖膜相比，复合膜具有较低的杨氏模量和较高的断裂伸长率，特别是在湿态下。所有复合膜均为亲水性材料，与水的接触角在55°~ 65°之间。ELISA结果表明，纤维连接蛋白油复合膜的吸附量明显高于壳聚糖膜。采用pc12细胞培养法评价材料对神经细胞的亲和力。在60wt%明胶复合膜上培养的细胞比壳聚糖膜上培养的细胞分化更快，神经突延长。结果表明，与壳聚糖相比，壳聚糖与明胶的柔软弹性复合物具有更好的神经细胞亲和性，是一种有前景的神经再生候选生物材料。2003爱思唯尔科学有限公司版权所有。
A series of chitosan-gelatin composite films was prepared by varying the ratio of constituents. FT-IR and X-ray analysis showed good compatibility between these two biopolymers. Differential scanning calorimetry (DSC) analysis indicated that the water take-up of chitosan film increased when blended with gelatin. Composite film exhibited a lower Young's modulus and a higher percentage of elongation-at-break compared with chitosan film, especially in wet state. All composite films were hydrophilic materials with water contact angles ranging from 55degrees to 65degrees. The results obtained from ELISA indicated the adsorption amount of fibronectin oil composite films was much higher than on chitosan film. PC 12 cells culture was used to evaluate the nerve cell affinity of materials. The cells cultured on the composite film with 60wt% gelatin differentiated more rapidly and extended longer neurites than on chitosan film. The results suggest that the soft and elastic complex of chitosan and gelatin, which has better nerve cell affinity compared to chitosan, is a promising candidate biomaterial for nerve regeneration. (C) 2003 Elsevier Science Ltd. All rights reserved.