Determination of stability of the DNA double helix in an aqueous medium
Determination of stability of the DNA double helix in an aqueous medium
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水介质中 DNA 双螺旋稳定性的测定
DOI：
10.1002/bip.1969.360080502
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发表时间：
1969
期刊：
影响因子：
2.9
通讯作者：
T. M. Birshtein
中科院分区：
文献类型：
--
作者：
P. Privalov;O. Ptitsyn;T. M. Birshtein
本文讨论了利用DNA从天然状态到变性状态转变的热ΔH的量热数据确定天然DNA结构稳定化自由能Δ G_0的可能性。给出了T2噬菌体DNA在不同pH值和离子强度下的变性热的微量热测量结果。得到了不同条件下DNA天然结构稳定化自由能Δ G_0值。结果表明，在接近生理条件下，Δ G 0接近每碱基对1200卡/摩尔。
The possibility of determining the free energy of stabilization ΔG0 of native DNA structure with the help of calorimetric data on heats ΔH of transition from the native to denaturated state is considered. Results of microcalorimetric measurements of heats of denaturation of T2 phage DNA at, different values of pH and ionic strength of solution are given. Values of free energy of stabilization of the DNA native structure ΔG0 under various conditions have been obtained. It is shown that under conditions close to physiological ΔG0 approaches 1200 cal/mole per base pair.