Experimental challenges of sense codon reassignment: An innovative approach to genetic code expansion
Experimental challenges of sense codon reassignment: An innovative approach to genetic code expansion
复制标题
DOI：
10.1016/j.febslet.2013.11.039
复制
发表时间：
2014-01-31
期刊：
影响因子：
3.5
通讯作者：
Ling, Jiqiang
中科院分区：
文献类型：
--
作者：
Krishnakumar, Radha;Ling, Jiqiang
目前，通过掺入非规范氨基酸向蛋白质添加新的、多功能的化学和生物特性主要是通过终止密码子抑制来实现的。然而，找到新的“空白”密码子以增加此类插入的多样性和效率至关重要，从而将“有义密码子重新编码”带到遗传密码扩展领域的中心舞台。目前的乐观观点表明，使用吡咯赖氨酸系统与基因组信息的重新合成相结合，以实现有义密码子的重新分配。在回顾最近研究报告的严重实验挑战后，我们认为该领域的成功将取决于基因组的重新合成，但还涉及“重新布线”蛋白质合成及其质量控制的机制 (C) 2013 年欧洲生物化学协会联合会出版，Elsevier B.V. 保留所有权利。
The addition of new and versatile chemical and biological properties to proteins pursued through incorporation of non-canonical amino acids is at present primarily achieved by stop codon suppression. However, it is critical to find new "blank'' codons to increase the variety and efficiency of such insertions, thereby taking 'sense codon recoding' to center stage in the field of genetic code expansion. Current thought optimistically suggests the use of the pyrrolysine system coupled with re-synthesis of genomic information towards achieving sense codon reassignment. Upon review of the serious experimental challenges reported in recent studies, we propose that success in this area will depend on the re-synthesis of genomes, but also on 'rewiring' the mechanism of protein synthesis and of its quality control. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.