MicroRNA-191 regulates differentiation and migration of mesenchymal stem cells and their paracrine effect on angiogenesis
MicroRNA-191 regulates differentiation and migration of mesenchymal stem cells and their paracrine effect on angiogenesis
复制标题
DOI：
10.1007/s10529-020-02907-z
复制
发表时间：
2020-05
影响因子：
2.7
通讯作者：
Yuanxin Liu;Xi Liu;P. Ye;Xiafei Zhang;A. Schilling;T. Yonezawa;Guifang Gao;Xiaofeng Cui
中科院分区：
文献类型：
--
作者：
Yuanxin Liu;Xi Liu;P. Ye;Xiafei Zhang;A. Schilling;T. Yonezawa;Guifang Gao;Xiaofeng Cui
microRNAs（miRNAs）是器官发育的重要调控因子。其中，已知miR-191在早期胚胎发生中受到调节，而在癌症中失调。这种在未分化组织中的作用表明miR-191也可能在骨髓来源的间充质干细胞（BMSC）生理学中起作用。在这里，我们报告了miR-191降低MMP的表达和BMSCs的迁移。过表达miR-191的BMSCs条件培养基可阻断VEGF表达，抑制HUVECs血管生成。在成骨培养条件下，miR-191的抑制显著诱导骨形成。此外，我们的研究表明miR-191可能通过直接靶向CCAAT增强结合蛋白β（CEBPB）影响BMSCs的软骨形成。综上所述，我们证明了miR-191在BMSCs的分化、迁移和旁分泌功能中的作用。
MicroRNAs (miRNAs) are critical regulators in organ development. Among them, miR-191 is known to be regulated in early embryogenesis and dysregulated in cancer. This role in undifferentiated tissues suggests a possible part of miR-191 also in bone marrow derived mesenchymal stem cells (BMSCs) physiology. Here, we report that miR-191 decreased MMP expression and migration of BMSCs. Conditioned media of miR-191 overexpressing BMSCs block VEGF expression, and inhibit angiogenesis of HUVECs. Under osteogenic culture conditions, inhibition of miR-191 significantly induces bone formation. Moreover, our studies showed miR-191 might influence chondrogenesis of BMSCs by directly targeting CCAAT Enhancer Binding Protein Beta (CEBPB). Taken together, here we demonstrate the role of miR-191 in differentiation, migration and paracrine function of BMSCs.