Rat liver NAD(P)H:quinone oxidoreductase: cDNA expression and site-directed mutagenesis.
Rat liver NAD(P)H:quinone oxidoreductase: cDNA expression and site-directed mutagenesis.
复制标题
大鼠肝脏 NAD(P)H：醌氧化还原酶：cDNA 表达和定点诱变。
DOI：
10.1016/0006-291x(90)92006-l
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Chen,SA
中科院分区：
文献类型：
--
作者：
Forrest,GL;Qian,J;Ma,JX;Kaplan,WD;Akman,S;Doroshow,J;Chen,SA
克隆了大鼠肝脏NAD（P）H：醌氧化还原酶cDNA，并在含有巨细胞病毒（CMV）启动子的真核细胞表达质粒中表达。在COS7细胞中测量酶活性和RNA转录的瞬时表达。所表达的醌还原酶具有与大鼠肝酶相似的动力学性质，并且被双香豆素（一种已知的NAD（P）H：醌氧化还原酶抑制剂）抑制。使用该表达系统进行的定点诱变实验揭示了参与NAD（P）H结合的可能区域。
Rat liver NAD(P)H:quinone oxidoreductase cDNA was cloned and expressed in a eukaryotic cell expression plasmid containing a cytomegalovirus (CMV) promoter. Transient expression of enzyme activity and RNA transcription were measured in COS7 cells. The expressed quinone reductase has kinetic properties similar to the rat liver enzyme and is inhibited by dicourmarol, a known inhibitor of NAD(P)H:quinone oxidoreductase. Site-directed mutagenesis experiments carried out using this expression system revealed possible regions involved in NAD(P)H binding.