Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses.
Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses.
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DOI：
10.3390/ph9040078
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发表时间：
2016-12-16
期刊：
影响因子：
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通讯作者：
García-Sacristán A
中科院分区：
文献类型：
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作者：
González VM;Martín ME;Fernández G;García-Sacristán A
正确的诊断是治疗病毒性疾病的关键因素。时间是流感、埃博拉和SARS等迅速发展和流行病学危险疾病的最重要因素。慢性病毒性疾病，如HIV-1或HCV是无症状或少症状的，治疗的成功主要取决于感染因子的早期检测。在过去的几年中，适体技术已被用于广泛的诊断和治疗应用，具体地说，目前正在探索使用针对病毒蛋白的适体的几种策略。从诊断的角度来看，适体被设计为诊断系统中的生物识别元件，以检测血液（血清或血浆）中或感染细胞中的病毒蛋白。适体的另一个潜在用途是用于病毒感染的治疗，使用靶向宿主细胞基质受体的适体干扰病毒和宿主之间的相互作用，或在细胞内攻击病毒，靶向病毒复制周期中涉及的蛋白质。在本文中，我们回顾了如何发现针对病毒蛋白质的适体，重点是最近的进展，提高适体的性能作为一个真实的工具，用于病毒感染的检测和治疗。
Appropriate diagnosis is the key factor for treatment of viral diseases. Time is the most important factor in rapidly developing and epidemiologically dangerous diseases, such as influenza, Ebola and SARS. Chronic viral diseases such as HIV-1 or HCV are asymptomatic or oligosymptomatic and the therapeutic success mainly depends on early detection of the infective agent. Over the last years, aptamer technology has been used in a wide range of diagnostic and therapeutic applications and, concretely, several strategies are currently being explored using aptamers against virus proteins. From a diagnostics point of view, aptamers are being designed as a bio-recognition element in diagnostic systems to detect viral proteins either in the blood (serum or plasma) or into infected cells. Another potential use of aptamers is for therapeutics of viral infections, interfering in the interaction between the virus and the host using aptamers targeting host-cell matrix receptors, or attacking the virus intracellularly, targeting proteins implicated in the viral replication cycle. In this paper, we review how aptamers working against viral proteins are discovered, with a focus on recent advances that improve the aptamers’ properties as a real tool for viral infection detection and treatment.