Comparative Analysis of Cellular and Growth Factor Composition in Bone Marrow Aspirate Concentrate and Platelet-Rich Plasma.
Comparative Analysis of Cellular and Growth Factor Composition in Bone Marrow Aspirate Concentrate and Platelet-Rich Plasma.
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DOI：
10.1155/2018/1549826
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Mishima H
中科院分区：
文献类型：
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作者：
Sugaya H;Yoshioka T;Kato T;Taniguchi Y;Kumagai H;Hyodo K;Ohneda O;Yamazaki M;Mishima H
本研究的目的是使用我们实验室建立的方案定量从全血制备的骨髓抽吸浓缩物（BMAC）和富血小板血浆（PRP）中的干细胞和生长因子（GF）含量。我们检查了2015年1月至2015年6月在我院接受自体BMAC移植治疗的10例股骨头坏死患者。我们通过流式细胞术定量了BMAC和PRP中的CD 34+和CD 31 − CD 45 − CD 90 + CD 105+细胞。此外，我们使用酶联免疫吸附测定法和统计学分析测定了BMAC和PRP中的各种GF，即碱性成纤维细胞生长因子（b-FGF）、血小板衍生生长因子-BB（PDGF-BB）、血管内皮生长因子（VEGF）、转化生长因子-β1（TGF-β1）和骨形态发生蛋白-2（BMP-2）。CD 34+和CD 31 −45−90+105+细胞约占BMAC中细胞的1.9%和0.03%，PRP中无细胞。BMAC中b-FGF的含量高于PRP（P < 0.001），而PDGF-BB、VEGF、TGF-β1和BMP-2的含量在两种标本中无显著差异。BMAC平均含有1.9%的CD 34+和0.03%的CD 31 −45−90+105+细胞，b-FGF水平高于PRP。
The purpose of this study was to quantify the stem cell and growth factor (GF) contents in the bone marrow aspirate concentrate (BMAC) and platelet-rich plasma (PRP) prepared from whole blood using a protocol established in our laboratory. We examined 10 patients with osteonecrosis of the femoral head who were treated by autologous BMAC transplantation at our hospital between January 2015 and June 2015. We quantified CD34+ and CD31−CD45−CD90+CD105+ cells in BMAC and PRP by flow cytometry. Additionally, we measured various GFs, that is, basic fibroblast growth factor (b-FGF), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1), and bone morphogenetic protein-2 (BMP-2) in BMAC and PRP using enzyme-linked immunosorbent assays and statistical analyses. CD34+ and CD31−45−90+105+ cells accounted for approximately 1.9% and 0.03% of cells in BMAC and no cells in PRP. The concentration of b-FGF was higher in BMAC than in PRP (P < 0.001), whereas no significant differences in the levels of PDGF-BB, VEGF, TGF-β1, and BMP-2 were observed between the two types of sample. BMAC had an average of 1.9% CD34+ and 0.03% CD31−45−90+105+ cells and higher levels of b-FGF than those of PRP.