Enhanced glucan formation of filamentous fungi by effective mixing, oxygen limitation and fed-batch processing
Enhanced glucan formation of filamentous fungi by effective mixing, oxygen limitation and fed-batch processing
复制标题
DOI：
10.1007/bf01576364
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
U. Rau;E. Gura;E. Olszewski;F. Wagner
中科院分区：
文献类型：
--
作者：
U. Rau;E. Gura;E. Olszewski;F. Wagner
研究了裂褶菌和葡萄糖小核菌葡聚糖的形成。提供了分批培养期间获得的工艺数据。葡聚糖的释放可以通过氧限制来改善。因此，氧气以相反的方式影响生长和葡聚糖释放。这种氧依赖性调节的可能途径进行了讨论。试验了导流筒/螺旋桨系统、拉什顿涡轮、风扇和螺带式叶轮以及底桨和中间桨搅拌器。d *=0.64的4叶片风扇叶轮产生最佳结果，因为对于该系统，有效的整体混合比气泡破裂（微观混合）重要得多。补料分批培养总是导致比分批过程更高的葡聚糖形成速率。
Glucan formation ofSchizophyllum communeandSclerotium glucanicumwere investigated. Process data obtained during batch cultivation are presented. Glucan release can be improved by oxygen limitation. Thus, growth and glucan release are influenced by oxygen in opposite ways. Possible pathways of this oxygen-dependent regulation are discussed. A draft-tube/propeller system, rushtonturbine-, fan- and helicon-ribbon-impeller as well as a fundaspi and intermig agitator were tested. The 4-bladed fan impeller withd*=0.64 yielded the best results, since effective bulk mixing is much more important than bubble break up (micromixing) with regard to this system. Fed-batch cultivation always resulted in higher rates of glucan formation than the batch process.