Multiple in vivo liver biopsies using a freeze-clamping technique
Multiple in vivo liver biopsies using a freeze-clamping technique
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DOI：
10.1080/08941930290085859
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发表时间：
2002-03-01
影响因子：
1.9
通讯作者：
Wittnich, C
中科院分区：
文献类型：
--
作者：
Belanger, MP;Askin, N;Wittnich, C
开发了一种多重体内肝活检技术，用于测量不稳定的代谢物（创造性磷酸盐[CP]，ATP，乳酸盐），而不干扰正常的灌注和代谢。麻醉大鼠（n = 7）进行腹部中线切口以暴露肝脏。在20分钟内进行了4次活检。通过活检近端的小型非创伤性夹钳控制出血。预冻敷料钳抓住肝脏，剪刀剪下活检组织，活检组织在液氮中冷冻。出血极少，血流动力学保持稳定。这种技术并发症少，出血容易控制，并且允许进行大量的多个活检，这提供了可靠的代谢数据，并且可以持续地进行教学。
A multiple in vivo liver biopsy technique was developed to measure labile metabolites (creative phosphate [CP], ATP, lactate) without interfering with normal perfusion and metabolism. Anesthetized rats (n = 7) had a midline abdominal incision done to expose the liver. Four biopsies were taken across 20 min. Bleeding was controlled by a small, nontraumatic clamp proximal to the biopsy. Prefrozen dressing forceps grasped the liver and scissors cut the biopsy, which was frozen in liquid nitrogen. Bleeding was minimal and hemodynamic stability was preserved. This technique has few complications, bleeding is easily controlled, and it allows for large multiple biopsies, which give reliable metabolic data and can be consistently taught.