Specific pathway selection by the early projections of individual peripheral sensory neurons in the embryonic medicinal leech.
Specific pathway selection by the early projections of individual peripheral sensory neurons in the embryonic medicinal leech.
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胚胎药用水蛭中个体外周感觉神经元的早期预测的特定途径选择。
DOI：
10.1002/neu.480251002
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
J. Johansen
中科院分区：
文献类型：
--
作者：
J. Jellies;K. Johansen;J. Johansen
In leech, the central annulus of each midbody segment possesses seven pairs of sensilla, which are mixed clusters of primary peripheral sensory neurons that extend their axons into the CNS where they segregate into distinct fascicles. Pathway selection by individual afferent growth cones of sensillar neurons was examined by double labeling using intracellular dye-filling with antibody labeling in early Hirudo medicinalis embryos. The monoclonal antibody Lan3-2 was used because sensillar neuronal tracts are specifically labeled by this antibody. Examining 68 individually filled neurons we found that sensillar neuron growth cones bifurcate within the CNS, that they project long filopodia capable of sampling the local environment, and that all of them appeared to choose a single particular CNS fascicle without apparent retraction or realignment of growth cones. Furthermore, each side of the bifurcating afferent growth cones always chose the same fascicle, implying a specific choice of a distinct labeled pathway. By dye-filling individual central neurons (P-cells), we show that there are centrally projecting axons present at the time sensillar afferents enter the ganglionic primordia and select a particular fascicle, and we confirm that at least the dorsal peripheral nerve is likely to be pioneered by central neurons, not by the peripheral afferents. In the sensillum studied here, we found examples of sensory neurons extending axons into one of all the available fascicles. Thus, an individual embryonic sensillum possesses a heterogeneous population of afferents with respect to the central fascicle chosen. This is consistent with the idea that segregation into distinct axon fascicles may be based upon functional differences between individual afferent neurons. Our findings argue strongly in favor of specific pathway selection by afferents in this system and are consistent with previous suggestions that there exists a hierarchy of cues, including surface glycoconjugates that mediate navigation of the sensillar growth cones and the fasciculation of their axons.
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影响因子：
2.7
作者：
Torrence,SA;Law,MI;Stuart,DK
通讯作者：
Stuart,DK
通过去除糖基磷脂酰肌醇锚定的细胞表面蛋白来破坏体内先锋生长锥的引导。
DOI：
10.1242/dev.114.2.507
发表时间：
1992
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Chang,WS;Serikawa,K;Allen,K;Bentley,D
通讯作者：
Bentley,D
发育中的大脑皮层中先驱和追随生长锥的形态。
DOI：
10.1002/neu.480220608
发表时间：
1991
期刊：
Journal of neurobiology
影响因子：
--
作者：
Kim,GJ;Shatz,CJ;McConnell,SK
通讯作者：
McConnell,SK
胚胎生殖管的间充质指导药用水蛭中 Retzius 神经元的生长过程。
影响因子：
2.7
作者：
French,KA;Jordan,SM;Loer,CM;KristanJr,WB
通讯作者：
KristanJr,WB
分层指导线索和选择性轴突通路形成。
影响因子：
--
作者：
Johansen,J;Johansen,KM;Briggs,KK;Kopp,DM;Jellies,J
通讯作者：
Jellies,J