Stress-induced pyruvate accumulation contributes to cross protection in a fungus
Stress-induced pyruvate accumulation contributes to cross protection in a fungus
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应激诱导的丙酮酸积累有助于真菌的交叉保护
DOI：
10.1111/1462-2920.14058
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发表时间：
2018
影响因子：
5.1
通讯作者：
Fang Weiguo
中科院分区：
文献类型：
--
作者：
Zhang Xing;Leger Raymond J. St.;Fang Weiguo
通常观察到，微生物受到温和的应力不仅对较高剂量的相同应力产生耐受性，而且对其他应力也产生耐受性-这种现象称为交叉保护。交叉保护的机制尚未完全揭示。在这里，我们报告，热休克诱导交叉保护，对紫外线，氧化和渗透/盐胁迫条件下的世界性真菌绿僵菌罗伯茨。类似地，氧化和渗透/盐胁迫也诱导了针对多种其他胁迫的交叉保护。我们发现，氧化和渗透/盐胁迫产生丙酮酸的积累，清除应激诱导的活性氧并促进真菌生长。因此，应激诱导的丙酮酸积累有助于交叉保护。RNA-seq和qRT-PCR分析表明，UV、渗透/盐和氧化应激条件降低了三氯乙酸循环和发酵途径中丙酮酸消耗基因的表达水平，导致丙酮酸积累。我们的工作提出了一种新的微生物交叉保护机制。
It is commonly observed that microorganisms subjected to a mild stress develop tolerance not only to higher doses of the same stress but also to other stresses – a phenomenon called cross protection. The mechanisms for cross protection have not been fully revealed. Here, we report that heat shock induced cross protection against UV, oxidative and osmotic/salt stress conditions in the cosmopolitan fungusMetarhizium robertsii. Similarly, oxidative and osmotic/salt stresses also induced cross protection against multiple other stresses. We found that oxidative and osmotic/salt stresses produce an accumulation of pyruvate that scavenges stress‐induced reactive oxygen species and promotes fungal growth. Thus, stress‐induced pyruvate accumulation contributes to cross protection. RNA‐seq and qRT‐PCR analyses showed that UV, osmotic/salt and oxidative stress conditions decrease the expression level of pyruvate consumption genes in the trichloroacetic acid cycle and fermentation pathways leading to pyruvate accumulation. Our work presents a novel mechanism for cross protection in microorganisms.