A large noncoding RNA is a marker for murine hepatocellular carcinomas and a spectrum of human carcinomas
A large noncoding RNA is a marker for murine hepatocellular carcinomas and a spectrum of human carcinomas
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DOI：
10.1038/sj.onc.1209846
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发表时间：
2007-02-08
期刊：
影响因子：
8
通讯作者：
Edgington, T. S.
中科院分区：
文献类型：
--
作者：
Lin, R.;Maeda, S.;Edgington, T. S.
肿瘤标志物有助于理解肿瘤的分子细胞生物学，为肿瘤的诊断和干预提供潜在的靶点。我们在这里发现了一个新的小鼠基因，肝素（hcn），编码一个7 kb的mrna样转录物。该基因似乎是人类α基因MALAT-1的鼠同源物。该基因及其同源物缺乏可靠的开放阅读框，与高度保守的大型非编码RNA （ncRNA）一致。在所有的前致癌物诱导的小鼠肝细胞癌（HCCs）和人肝细胞癌结节中，与未受损伤的肝脏相比，表达明显升高。定量分析表明，hcc中RNA水平比未累及的肝脏高6-7倍，这使其成为一种感兴趣的分子。该ncRNA在分析的所有五种非肝脏人类癌中均过表达，与肿瘤细胞的潜在标记物和肿瘤分子细胞生物学的潜在参与者一致。
Tumor markers can facilitate understanding molecular cell biology of neoplasia and provide potential targets for the diagnosis and insight for intervention. We here identify a novel murine gene, hepcarcin (hcn), encoding a 7-kb mRNA-like transcript. The gene appears to be the murine ortholog of the human alpha gene, that is, MALAT-1. The gene and homologs lack credible open reading frames, consistent with a highly conserved large noncoding RNA (ncRNA). In all nodules of procarcinogen-induced murine hepatocellular carcinomas (HCCs) and human HCCs, expression was markedly elevated compared to the uninvolved liver. Quantitative analyses indicated a 6-7-fold increased RNA level in HCCs versus uninvolved liver, advancing this as a molecule of interest. This ncRNA was overexpressed in all five non-hepatic human carcinomas analysed, consistent with a potential marker for neoplastic cells and potential participant in the molecular cell biology of neoplasia.