P4HB modulates epithelial-mesenchymal transition and the β-catenin/Snail pathway influencing chemoresistance in liver cancer cells
P4HB modulates epithelial-mesenchymal transition and the β-catenin/Snail pathway influencing chemoresistance in liver cancer cells
复制标题
P4HB 调节上皮间质转化和影响肝癌细胞化疗耐药性的 β-catenin/Snail 通路
DOI：
10.3892/ol.2020.11569
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发表时间：
2020-07-01
期刊：
影响因子：
2.9
通讯作者：
Xia, Wei
中科院分区：
文献类型：
--
作者：
Ma, Xing;Wang, Jiening;Xia, Wei
本研究旨在探讨脯氨酰4-羟化酶β多肽（P4 HB）在肝癌化疗耐药中的作用。用阿霉素处理和筛选耐药肝癌细胞系，如HepG 2/阿霉素（ADR）细胞。用基因干扰法沉默肝癌细胞P4 HB的表达。分别使用CCK 8、Transwell和伤口愈合测定来评估细胞活力、侵袭性和迁移。此外，使用逆转录-定量PCR和蛋白质印迹分析上皮-间质转化（EMT）和β-连环蛋白/Snail通路中关键基因和蛋白质的变化。成功地培养了耐药HepG 2/ADR细胞，HepG 2/ADR和HepG 2细胞系对ADR的IC（50）分别为4.85和0.61 μ M。HepG 2/ADR细胞的侵袭和迁移能力明显高于HepG 2细胞（P
The aim of the present study was to investigate the role of prolyl 4-hydroxylase beta polypeptide (P4HB) in the chemoresistance of liver cancer. Drug-resistant liver cancer cell lines, such as HepG2/adriamycin (ADR) cells, were treated and screened using adriamycin. Gene interference was used to silence the expression ofP4HBin liver cancer cells. Cell viability, invasiveness and migration were assessed using CCK8, Transwell and wound healing assays, respectively. In addition, changes to key genes and proteins in the epithelial-mesenchymal transition (EMT) and beta-catenin/Snail pathway were analyzed using reverse transcription-quantitative PCR and western blotting. Drug-resistant HepG2/ADR cells were successfully cultivated; the IC(50)to ADR for HepG2/ADR and HepG2 cell lines was 4.85 and 0.61 mu M, respectively. HepG2/ADR cells exhibited higher invasion and migration abilities compared with HepG2 cells (P