Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance
Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance
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DOI：
10.1073/pnas.95.11.6531
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发表时间：
1998-05-26
影响因子：
11.1
通讯作者：
Dong, XN
中科院分区：
文献类型：
--
作者：
Cao, H;Li, X;Dong, XN
最近克隆的拟南芥NPR1基因是获得性抗性反应的关键调节因子，在诱导后，NPR1表达升高，NPR1蛋白被激活，进而诱导一系列下游发病机制相关基因的表达。在这项研究中，我们发现 NPR1 以剂量依赖性方式赋予对病原体丁香假单胞菌和寄生霜霉的抗性。 NPR1 的过度表达会增强抗性，但对植物没有明显的有害影响。因此，首次证明单个基因是植物非特异性抗性基因工程的可行靶标。
The recently cloned NPR1 gene of Arabidopsis thaliana is a key regulator of acquired resistance responses, Upon induction, NPR1 expression is elevated and the NPR1 protein is activated, in turn inducing expression of a battery of downstream pathogenesis-related genes. In this study, we found that NPR1 confers resistance to the pathogens Pseudomonas syringae and Peronospora parasitica in a dosage-dependent fashion. Overexpression of NPR1 leads to enhanced resistance with no obvious detrimental effect on the plants. Thus, for the first time, a single gene is shown to be a workable target for genetic engineering of nonspecific resistance in plants.