ECDYSTEROID LEVELS AND DEVELOPMENTAL EVENTS DURING LARVAL MOLTING IN THE SILKWORM, BOMBYX-MORI
ECDYSTEROID LEVELS AND DEVELOPMENTAL EVENTS DURING LARVAL MOLTING IN THE SILKWORM, BOMBYX-MORI
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DOI：
10.1016/0022-1910(81)90072-x
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发表时间：
1981-01-01
影响因子：
2.2
通讯作者：
AGUI, N
中科院分区：
文献类型：
--
作者：
KIGUCHI, K;AGUI, N
在家蚕中，B。在家蚕中，气孔的顶裂是幼虫蜕皮开始的第一个可见迹象。气门顶裂后，通过旧气门周围的角质层，可以很容易地识别新气门形成的特征序列。这个序列，称为气孔指数，被用作幼虫蜕皮期精确发育年表的基础。在一定的气门指数阶段，在幼虫腹侧腹珠被的组织学变化也进行了检查。通过allatectomy和腹部结扎，保幼激素和蜕皮激素的分泌的关键时期，分别是在开始的气门apolysis和时，当一般apolysis完成。通过放射免疫测定法测量的血淋巴蜕皮激素水平为. apprx。4龄前2天为30-50 ng/ml，气门顶裂开始时为60-70 ng/ml，全身顶裂完成时为200 ng/ml。激素的最大水平为. apprx。蜕皮中期为290 ng/ml。文中还讨论了蜕皮激素滴度与幼虫蜕皮过程中形态学变化的关系。
In the silkworm, B. mori, apolysis of the spiracle is the 1st visible sign of the initiation of a larval molt. After spiracular apolysis, the characteristic sequence of new spiracle formation is recognized easily through the cuticle around the old spiracle. This sequence, called the spiracle index, was used as the basis for a precise development chronology of the larval molting period. At certain spiracle-index stages, histological changes in the larval ventral abdominal integument were also examined. By allatectomy and abdominal ligations, the critical periods for the secretion of juvenile hormone and ecdysone, respectively, were at the initiation of spiracular apolysis and at the time when general apolysis was complete. The hemolymph-ecdysteroid level as measured by radioimmunoassay was .apprx. 30-50 ng/ml during the first 2 days of the 4th instar, 60-70 ng/ml at the time of initiation of spiracular apolysis and 200 ng/ml at the time of completion of apolysis of the general body surface. The maximal level of the hormone was .apprx. 290 ng/ml at the mid-molting period. The relationship between the ecdysteroid titer and morphological events occurring during the larval molt was discussed.