CYTOSKELETAL DYNAMICS IN LIVING PLANT-CELLS
CYTOSKELETAL DYNAMICS IN LIVING PLANT-CELLS
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DOI：
10.1006/cbir.1993.1050
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发表时间：
1993-02-01
影响因子：
3.9
通讯作者：
ZHANG, DH
中科院分区：
文献类型：
--
作者：
HEPLER, PK;CLEARY, AL;ZHANG, DH
微管和微丝已在活植物细胞中成像，并记录了它们在分裂、生长和发育过程中的动态变化。羧基荧光素标记的脑微管蛋白已被注射到在显微镜台上的培养室中保持活跃状态的细胞中。随后用共焦显微镜成像揭示了前期带、有丝分裂器、成膜体和皮质阵列中的微管。在过渡阶段观察到这些微管的结构变化。此外，它们的动态特征通过在高钙、低温和安磺林药物中的解聚以及恢复正常条件时的再聚合来证明。对活的紫露草雄蕊毛细胞进行检查，这些毛细胞已注射荧光鬼笔环肽以标记肌动蛋白微丝，揭示了迄今为止未公开的分裂位点的制备和成膜体细胞骨架的动力学。在前期，微丝遍布整个细胞皮层，而前期带区域的微丝则横向排列。在核膜破裂时，它们会特异性地分解，留下一个圆周区域，在整个分裂过程中，微丝在该区域中保持不存在。在胞质分裂期间，微丝在成膜体中产生，方向与微管平行，但排除在 MT 重叠和细胞板囊泡聚集的区域之外。成膜体微丝以类似于微管的方式，长度缩短，周长扩大，并最终在细胞板完成时分解。
Microtubules and microfilaments have been imaged in living plant cells and their dynamic changes recorded during division, growth and development. Carboxyfluorescein labeled brain tubulin has been injected into cells that are maintained in an active state in a culture chamber on the microscope stage. Subsequent imaging with the confocal microscope reveals microtubules in the preprophase band, the mitotic apparatus, the phragmoplast, and the cortical array. The structural changes of these microtubules have been observed during transitional stages. In addition, their dynamic features are demonstrated by depolymerization in elevated calcium, low temperature, and in the drug oryzalin, and by repolymerization when returned to normal conditions. Examination of living Tradescantia stamen hair cells, which have been injected with fluorescent phalloidin to label the actin microfilaments, reveals hitherto undisclosed aspects of the preparation of the division site and dynamics of the phragmoplast cytoskeleton. During prophase microfilaments occur throughout the cell cortex, with those in the region of the preprophase band becoming transversely aligned. At nuclear envelope breakdown, these specifically disassemble, leaving a circumferential zone from which microfilaments remain absent throughout division. During cytokinesis microfilaments arise within the phragmoplast, oriented parallel to the microtubules, but excluded from the zone where the MTs overlap and where cell plate vesicles aggregate. The phragmoplast microfilaments, in a manner similar to microtubules, shorten in length, expand in girth, and eventually disassemble when the cell plate is complete.