A bacterial chemoreceptor that mediates chemotaxis to two different plant hormones.
A bacterial chemoreceptor that mediates chemotaxis to two different plant hormones.
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DOI：
10.1111/1462-2920.15920
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发表时间：
2022-08
影响因子：
5.1
通讯作者：
Matilla, Miguel A.
中科院分区：
文献类型：
--
作者：
Rico-Jimenez, Miriam;Roca, Amalia;Krell, Tino;Matilla, Miguel A.
吲哚 - 3 - 乙酸（IAA）是主要的天然生长素，由所有生命界的生物产生。除了调节植物生长和发育外，IAA通过调节细菌基因表达和生理机能，在植物与促生长细菌和植物病原细菌的相互作用中发挥重要作用。我们在此表明，一株能代谢IAA的植物相关恶臭假单胞菌分离株对IAA具有趋化性，且这种趋化性与生长素代谢无关。我们发现IAA趋化性基于PcpI化学受体的活性，并且pcpI的异源表达使不同环境和人类致病性的假单胞菌属菌株获得对IAA的趋化性。通过配体筛选、微量热法和定量趋化性测定，我们发现PcpI不能直接结合IAA，但能识别并介导对多种芳香族化合物的趋化吸引，包括植物激素水杨酸。还研究了pcpI的表达及其在与植物相互作用中的作用。PcpI扩展了化学受体所识别的中心信号分子的范围。据我们所知，这是关于一种细菌受体对两种不同植物激素作出反应的首次报道。我们的研究强化了IAA和水杨酸作为界内和界间信号分子的多功能作用。
Indole‐3‐acetic acid (IAA) is the main naturally occurring auxin and is produced by organisms of all kingdoms of life. In addition to the regulation of plant growth and development, IAA plays an important role in the interaction between plants and growth‐promoting and phytopathogenic bacteria by regulating bacterial gene expression and physiology. We show here that an IAA metabolizing plant‐associated Pseudomonas putida isolate exhibits chemotaxis to IAA that is independent of auxin metabolism. We found that IAA chemotaxis is based on the activity of the PcpI chemoreceptor and heterologous expression of pcpI conferred IAA taxis to different environmental and human pathogenic isolates of the Pseudomonas genus. Using ligand screening, microcalorimetry and quantitative chemotaxis assays, we found that PcpI failed to bind IAA directly, but recognized and mediated chemoattractions to various aromatic compounds, including the phytohormone salicylic acid. The expression of pcpI and its role in the interactions with plants was also investigated. PcpI extends the range of central signal molecules recognized by chemoreceptors. To our knowledge, this is the first report on a bacterial receptor that responds to two different phytohormones. Our study reinforces the multifunctional role of IAA and salicylic acid as intra‐ and inter‐kingdom signal molecules.
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影响因子：
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