Correction for Liu et al., "FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region".
Correction for Liu et al., "FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region".
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作者：
鞭毛马达蛋白FliL在许多微生物中是保守的，但其确切作用已被各种FliL突变表型所掩盖。我们重新分析了fliL研究的结果，发现他们利用的等位基因在保留的N端和C端区域的数量上不同。在不存在C-末端周质结构域的情况下保留N-末端胞质和跨膜螺旋（TM）区域的等位基因导致运动性的丧失，而完全缺乏N-末端区域的等位基因，独立于周质结构域，保留运动性。然后，我们在幽门螺杆菌中检验了这一预测，并找到了支持这一观点的证据。这一分析表明，FliL的功能在细菌中可能比以前认为的更为保守，它不是运动所必需的，并且N-末端区域具有调节运动功能的负面能力。重要的是，FliL是一种在细菌鞭毛运动中发现的蛋白质，但它的作用尚不清楚。为了研究FliL的功能，科学家们经常将其移除，看看会发生什么。FliL的丧失被认为具有取决于微生物的不同影响。然而，我们发现，部分混淆的出现是因为科学家无意中删除了蛋白质的不同部分。我们的分析和数据表明，留下N-末端区域阻断运动性，而完全去除FliL允许正常运动性。这一发现将有助于科学家理解FliL，因为它阐明了需要去除什么才能完全消除蛋白质，并且N末端区域可以阻止运动。
The flagellar motor protein FliL is conserved across many microbes, but its exact role has been obscured by varyingfliLmutant phenotypes. We reanalyzed results fromfliLstudies and found they utilized alleles that differed in the amount of N- and C-terminal regions that were retained. Alleles that retain the N-terminal cytoplasmic and transmembrane helix (TM) regions in the absence of the C-terminal periplasmic domain result in loss of motility, while alleles that completely lack the N-terminal region, independent of the periplasmic domain, retain motility. We then tested this prediction in Helicobacter pylorifliLand found support for the idea. This analysis suggests that FliL function may be more conserved across bacteria than previously thought, that it is not essential for motility, and that the N-terminal region has the negative ability to regulate motor function.IMPORTANCEFliL is a protein found in the flagellar motor of bacteria, but what it does was not clear. To study FliL function, scientists often remove it and see what happens. Loss of FliL was thought to have different effects depending on the microbe. We uncovered, however, that part of the confusion arose because scientists inadvertently removed different parts of the protein. Our analysis and data suggest that leaving the N-terminal regions blocks motility, while fully removing FliL allows normal motility. This finding will help scientists understand FliL because it clarifies what needs to be removed to fully eliminate the protein, and also that the N-terminal region can block motility.
影响因子：
6.4
作者：
Liu, Xiaolin;Roujeinikova, Anna;Ottemann, Karen M.
通讯作者：
Ottemann, Karen M.