Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells
Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells
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DOI：
10.1182/blood-2008-12-193888
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发表时间：
2009-07-09
期刊：
影响因子：
20.3
通讯作者：
Daley, George Q.
中科院分区：
文献类型：
--
作者：
McKinney-Freeman, Shannon L.;Naveiras, Olaia;Daley, George Q.
造血干细胞（HSC）表面抗原的前瞻性分离和表征。在这里，我们比较造血再生细胞的细胞表面表型从小鼠卵黄囊，卵巢-性腺-中肾，胎盘，胎肝，骨髓与来自小鼠胚胎干细胞（ESC-HSC）的体外分化的HSC。尽管c-Kit标记所有HSC群，但CD 41、CD 45、CD 34和CD 150是发育调节的：最早的胚胎HSC表达CD 41和CD 34，缺乏CD 45和CD 150，而更成熟的HSC缺乏CD 41和CD 34，表达CD 45和CD 150。ESC-HSC表达CD 41和CD 150，缺乏CD 34，并且对于CD 45是异质的。最后，虽然CD 48在所有检查的体内HSC中不存在，但ESC-HSC对于该分子的表达是异质的。这种独特的表型意味着发育不成熟的细胞群体具有原始和成熟HSC的特征。ESC-HSC的前瞻性分离将促进HSC成熟的研究，这些成熟对于受照射成人的正常功能植入至关重要。（血。2009; 114：268-278）
Surface antigens on hematopoietic stem cells (HSCs) enable prospective isolation and characterization. Here, we compare the cell-surface phenotype of hematopoietic repopulating cells from murine yolk sac, aorta-gonad-mesonephros, placenta, fetal liver, and bone marrow with that of HSCs derived from the in vitro differentiation of murine embryonic stem cells (ESC-HSCs). Whereas c-Kit marks all HSC populations, CD41, CD45, CD34, and CD150 were developmentally regulated: the earliest embryonic HSCs express CD41 and CD34 and lack CD45 and CD150, whereas more mature HSCs lack CD41 and CD34 and express CD45 and CD150. ESC-HSCs express CD41 and CD150, lack CD34, and are heterogeneous for CD45. Finally, although CD48 was absent from all in vivo HSCs examined, ESC-HSCs were heterogeneous for the expression of this molecule. This unique phenotype signifies a developmentally immature population of cells with features of both primitive and mature HSC. The prospective fractionation of ESC-HSCs will facilitate studies of HSC maturation essential for normal functional engraftment in irradiated adults. (Blood. 2009; 114: 268-278)