Protective action of the ginsenoside Rh3 in a rat myocardial ischemia-reperfusion injury model by inhibition of apoptosis induced via p38 mitogen-activated protein kinase/caspase-3 signaling.
Protective action of the ginsenoside Rh3 in a rat myocardial ischemia-reperfusion injury model by inhibition of apoptosis induced via p38 mitogen-activated protein kinase/caspase-3 signaling.
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DOI：
10.1177/0300060520969090
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Mao Y
中科院分区：
文献类型：
--
作者：
Cao L;Gao Y;Zhu J;Zhang J;Dong M;Mao Y
目的：探讨人参皂苷Rh3对大鼠心肌缺血再灌注(MIR)后caspase-3和p38丝裂原活化蛋白激酶(MAPK)信号转导通路的影响。15只雄性SD大鼠随机分为MIR组(My组，n = 5)、假手术组(SS组，n = 5)和人参皂苷Rh3组(GR组，n = 5)。与GR组和SS组相比，MY组显示最大的心肌梗死。GR组心肌细胞存活率明显高于My组，细胞凋亡率显著低于My组。GR组梗死组织纤维排列紊乱，但肿胀较轻，纤维取向较My组更有条理。与My组和SS组相比，GR组p-p38MAPK蛋白和caspase-3mRNA表达水平降低。Rh3通过抑制p38MAPK通路，从而抑制caspase-3参与细胞凋亡，从而显著改善心肌坏死和心肌组织中caspase-3的水平。因此，Rh3能有效地抑制心肌梗死后细胞凋亡途径的升高，有望成为救治心肌梗死的有效药物。
To investigate the protective effects of the ginsenoside Rh3 on rats subjected to myocardial ischemia-reperfusion (MIR) via its impact on caspase-3 and the p38 mitogen-activated protein kinase (MAPK) pathway. Fifteen male Sprague-Dawley rats were randomly categorized into the MIR group (MY group, n = 5), sham surgery group (SS group, n = 5), and ginsenoside Rh3 group (GR group, n = 5). The MY group exhibited the largest myocardial infarctions compared with the GR and SS groups. The GR group exhibited significantly higher cell viability of cardiomyocytes and significantly decreased apoptosis compared with the MY group. Fibrils of infarcted tissue in the GR group were disordered but less swollen, with a more organized fibril orientation than those in the MY group. The GR group showed reduced p-p38 MAPK protein and caspase-3 mRNA expression levels compared with the MY and SS groups. Rh3 significantly improved myocardial necrosis and caspase-3 levels in myocardial tissues by suppressing the p38 MAPK pathway, thereby inhibiting caspase-3 involvement in apoptosis. Thus, Rh3 was effective in inhibiting the escalated apoptotic pathway in myocardial infarction and can potentially serve as a useful therapeutic agent to rescue myocardial infarction.
DOI：
10.1177/0300060520959502
发表时间：
2020-12
期刊：
The Journal of international medical research
影响因子：
--
作者：
Zhang L;Hu M;Chen Y;Wang Y
通讯作者：
Wang Y