E3 ubiquitin ligase ATL61 acts as a positive regulator in abscisic acid mediated drought response in Arabidopsis
E3 ubiquitin ligase ATL61 acts as a positive regulator in abscisic acid mediated drought response in Arabidopsis
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E3泛素连接酶ATL61在拟南芥脱落酸介导的干旱反应中发挥正调节作用
DOI：
10.1016/j.bbrc.2020.05.067
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发表时间：
2020
影响因子：
3.1
通讯作者：
Yang Yi
中科院分区：
文献类型：
--
作者：
Yang Ruirui;Wang Tao;Shi Wensen;Li Siyu;Liu Zhibin;Wang Jianmei;Yang Yi
脱落酸（阿坝）是一种重要的植物激素，在植物生长发育过程中介导非生物胁迫。许多E3连接酶已被报道参与阿坝信号通路。在这项研究中，我们确定了一个C3H2C3环型E3连接酶，拟南芥Toxicos en Levadura 61（ATL 61），调节植物干旱胁迫。体外酶活性测定表明，ATL 61具有E3泛素连接酶活性，而点突变（H109A，H122A）（mATL 61）则使其E3泛素连接酶活性丧失。ATL 61过表达植株表现出阿坝敏感性，对干旱的耐受性更强，而atl61突变体植株对阿坝不敏感。此外，mATL 61过表达株系表现出与野生型（WT）植物相似的ABA相关表型。ABA介导的干旱胁迫相关基因RD20和RD22在ATL 61过表达植株中的转录丰度高于WT、atl61和mATL 61植株。我们的研究结果表明，ATL 61在ABA介导的干旱胁迫反应中起正调节作用。(C)2020爱思唯尔公司All rights reserved.
Abscisic acid (ABA) is an important plant hormone that mediates abiotic stresses in plant growth and development. A number of E3 ligases have been reported to be involved in ABA signaling pathway. In this study, we identified a C3H2C3 RING-type E3 ligase, Arabidopsis thaliana Toxicos en Levadura 61 (ATL61), which regulated drought stress in planta. Enzyme assay in vitro demonstrated that ATL61 had E3 ubiquitin ligase activity, while point mutation of ATL61(H109A, H122A) (mATL61) abolished its E3 ubiquitin ligase activity. ATL61 overexpression plants exhibited ABA hypersensitivity and were more tolerant to drought, while the atl61 mutant plants were insensitive to ABA. Moreover, mATL61 overexpression lines exhibited similar ABA-related phenotypes with wild type (WT) plants. The transcript abundances of ABA-mediated drought stress-related genes RD20 and RD22 were higher in ATL61 overexpression plants than those in WT, atl61, and mATL61 plants. Our results indicated that ATL61 acted as a positive regulator in the ABA-mediated drought stress response. (C) 2020 Elsevier Inc. All rights reserved.