Fabrication of layer-by-layer deposited multilayer films containing DNA and gold nanoparticle for norepinephrine biosensor
Fabrication of layer-by-layer deposited multilayer films containing DNA and gold nanoparticle for norepinephrine biosensor
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DOI：
10.1016/j.aca.2004.05.062
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发表时间：
2004-08-16
影响因子：
6.2
通讯作者：
Lin, XQ
中科院分区：
文献类型：
--
作者：
Lu, LP;Wang, SQ;Lin, XQ
本工作描述了电沉积DNA膜的制备和表征（与金纳米粒子的生物传感器的设计）。将金纳米颗粒沉积在DNA层表面，构建纳米电极阵列的混合装置。用原子力显微镜、扫描电镜和电化学阻抗谱对纳米金掺杂的DNA复合电极进行了表征。在抗坏血酸（AA）存在下，该电极成功地用于去甲肾上腺素（NE）的选择性测定。结果表明，NE的电极氧化反应的可逆性显著提高，使NE的伏安峰电位负移200 mV，峰电流大幅增加。通过在静态溶液中使用DPV获得5 nM NE的检测限。大量过量AA的共存不干扰检测。该电极具有良好的灵敏度、选择性和选择性。(C)2004 Elsevier B.V.保留所有权利。
The present work describes the preparation and characterization of an electrodeposited DNA membrane (loped with gold nanoparticles for the design of biosensors. The gold nanoparticles were deposited on the surface of DNA layer to build a hybrid device of nanoscale electrode array. The gold nanoparticles -doped DNA composite electrode was characterized by atomic force microscopy, scanning electron microscope, and electrochemistry involving electrochemical impedance spectroscopy. This electrode was successfully used for selective determination of norepinephrine (NE) in the presence of ascorbic acid (AA). The reversibility of the electrode oxidation reaction of NE is significantly improved in result of 200 mV negative shift of the voltammetric peak potential on the electrode, and a large increase in the peak current. A detection limit of 5 nM NE is obtained by using DPV in static solutions. The co-existence of a large excess of AA does not interfere with the detection. This electrode shows excellent sensitivity, good selectivity and antifouling properties. (C) 2004 Elsevier B.V. All rights reserved.