Analysis of differential proteomes in pathogenic and non‐pathogenic Leptospira: Potential pathogenic and virulence factors
Analysis of differential proteomes in pathogenic and non‐pathogenic Leptospira: Potential pathogenic and virulence factors
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DOI：
10.1002/pmic.200700855
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发表时间：
2009-07
期刊：
影响因子：
3.4
通讯作者：
V. Thongboonkerd;W. Chiangjong;Putita Saetun;S. Sinchaikul;Shui-Tein Chen;U. Kositanont
中科院分区：
文献类型：
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作者：
V. Thongboonkerd;W. Chiangjong;Putita Saetun;S. Sinchaikul;Shui-Tein Chen;U. Kositanont
钩端螺旋体病是由钩端螺旋体属螺旋体引起的一种细菌性人畜共患疾病。迄今为止，决定钩端螺旋体致病性和毒力的因素仍不清楚。我们进行了基于凝胶的蛋白质组分析，以评估致病性问号钩端螺旋体（澳大利亚血清型、布拉迪斯拉发型、秋季螺旋体和黄疸出血型）和非致病性双折钩体（Patoc血清型）中的差异钩端螺旋体蛋白质组。定量蛋白质组分析和 MS 蛋白质鉴定揭示了 33 种独特蛋白质的 42 种形式，其水平在致病性血清型中显着高于非致病性血清型。在四种致病血清型中，与其他三种致病性血清型相比，毒性更强的黄疸出血血清型（最常与患者的严重钩端螺旋体病相关）的 12 种独特蛋白质的 14 种形式的水平显着更高。其中一些已鉴定的蛋白质可能充当钩端螺旋体病的致病和/或毒力因子。
Leptospirosis is a bacterial zoonotic disease caused by spirochetes in the genus Leptospira. To date, factors determining the pathogenicity and virulence of leptospires remain unclear. We performed a gel‐based proteomic analysis to evaluate differential leptospiral proteomes in the pathogenic L. interrogans (serovars Australis, Bratislava, Autumnalis, and Icterohaemorrhagiae) and the non‐pathogenic L. biflexa (serovar Patoc). Quantitative proteome analysis and MS protein identification revealed 42 forms of 33 unique proteins whose levels were significantly greater in the pathogenic serovars compared with the non‐pathogenic serovar. Among the four pathogenic serovars, the more virulent serovar Icterohaemorrhagiae (which is most commonly associated with severe leptospirosis in patients) had significantly greater levels of 14 forms of 12 unique proteins, when compared with the other three pathogenic serovars. Some of these identified proteins may serve as the pathogenic and/or virulence factors of leptospirosis.