Immunocytological analysis of meiotic recombination in the American mink (Mustela vison)
Immunocytological analysis of meiotic recombination in the American mink (Mustela vison)
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DOI：
10.1111/j.1365-2052.2008.01808.x
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发表时间：
2009-04-01
期刊：
影响因子：
2.4
通讯作者：
Zhelezova, A. I.
中科院分区：
文献类型：
--
作者：
Borodin, P. M.;Basheva, E. A.;Zhelezova, A. I.
利用错配修复蛋白MLH1的免疫定位，我们估计了美国水貂(Mustela Vison)的遗传图谱的总长度、重组率和交叉分布。MLH1是一种错配修复蛋白，标记了突触膜复合体的交叉位点。我们从5只雄性水貂的130个精母细胞制备了分散的精细胞，并沿着1820个二价体定位了3320个MLH1焦点。雄性水貂基因组的总重组长度为1327 cM，基于所有染色体的MLH1焦点的平均数。总的重组速率估计为0.48 cm/Mb。在所有的二价体中，我们观察到MLH1焦点在远端附近有明显的峰，而在着丝粒附近有少量的峰。这表明，位于染色体近端区域的基因应该比位于端粒附近的物理上等距离的标记显示出更紧密的遗传连锁。
Using immunolocalization of MLH1, a mismatch repair protein that marks crossover sites along synaptonemal complexes, we estimated the total length of the genetic map, the recombination rate and crossover distribution in the American mink (Mustela vison). We prepared spreads from 130 spermatocytes of five male minks and mapped 3320 MLH1 foci along 1820 bivalents. The total recombination length of the male mink genome, based on the mean number of MLH1 foci for all chromosomes, was 1327 cM. The overall recombination rate was estimated to be 0.48 cM/Mb. In all bivalents, we observed prominent peaks of MLH1 foci near the distal ends and a paucity of them near the centromeres. This indicates that genes located at proximal regions of the chromosomes should display much tighter genetic linkage than physically equidistant markers located near the telomeres.