Spatially modulated fluorescence emission from moving particles
Spatially modulated fluorescence emission from moving particles
复制标题
DOI：
10.1063/1.3070536
复制
发表时间：
2009-01-26
影响因子：
4
通讯作者：
Johnson, Noble
中科院分区：
文献类型：
--
作者：
Kiesel, Peter;Bassler, Michael;Johnson, Noble
描述了一种用于流式细胞仪的光学检测技术，其在不使用精密光学元件的情况下提供高信噪比辨别，以使流式细胞仪能够结合高性能、坚固耐用、紧凑、低成本和易于使用。该使能技术被称为“空间调制发射”，并且当连续荧光的生物制品穿过用于光传输的预定图案时产生依赖时间的信号。将检测到的信号与已知模式相关实现了粒子信号与背景噪声的高分辨。这项技术通过对流经微流控芯片的荧光珠的测量进行了演示。
An optical detection technique for a flow cytometer is described, which delivers high signal-to-noise discrimination without precision optics to enable a flow cytometer that can combine high performance, robustness, compactness, low cost, and ease of use. The enabling technique is termed "spatially modulated emission" and generates a time-dependent signal as a continuously fluorescing bioparticle traverses a predefined pattern for optical transmission. Correlating the detected signal with the known pattern achieves high discrimination of the particle signal from background noise. The technique is demonstrated with measurements of fluorescent beads flowing through a microfluidic chip.