Carbon Nanotube Yarn Microelectrodes Promote High Temporal Measurements of Serotonin Using Fast Scan Cyclic Voltammetry
Carbon Nanotube Yarn Microelectrodes Promote High Temporal Measurements of Serotonin Using Fast Scan Cyclic Voltammetry
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DOI：
10.3390/s20041173
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发表时间：
2020-02-01
期刊：
影响因子：
3.9
通讯作者：
Zestos, Alexander G.
中科院分区：
文献类型：
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作者：
Mendoza, Alexander;Asrat, Thomas;Zestos, Alexander G.
四十多年来，碳纤维微电极 (CFME) 一直是神经递质检测的标准。然而，近年来，利用替代纳米材料通过快速扫描循环伏安法（FSCV）进行神经递质检测方面取得了许多进展。最近，碳纳米管（CNT）纱线已被开发为具有快速扫描循环伏安法的神经递质传感功能的工作电极材料。碳纳米管是神经递质检测的理想选择，因为它们具有较高的长径比，可实现单胺吸附和较低的检测限、更快的电子转移动力学以及对表面污染的抵抗力。几种用碳纳米管修饰 CFME 的方法提高了灵敏度，但也增加了噪音并导致不可重复的结果。在这项研究中，我们利用市售的碳纳米管纱线来制造微电极，作为血清素等神经递质的增强型神经递质传感器。 CNT 纱线微电极比 CFME 具有显着更高的灵敏度（循环伏安图的峰值氧化电流），并且通过峰值分离 (Delta(EP)) 值测量具有更快的电子转移动力学。此外，通过扫描速率和浓度实验测量，血清素和多巴胺都被吸附控制在电极表面。 CNT纱线微电极还可以在三十分钟内抵抗血清素在电极表面上的表面污染，并且对电极表面的灵敏度具有与波应用频率无关的响应。
Carbon fiber-microelectrodes (CFMEs) have been the standard for neurotransmitter detection for over forty years. However, in recent years, there have been many advances of utilizing alternative nanomaterials for neurotransmitter detection with fast scan cyclic voltammetry (FSCV). Recently, carbon nanotube (CNT) yarns have been developed as the working electrode materials for neurotransmitter sensing capabilities with fast scan cyclic voltammetry. Carbon nanotubes are ideal for neurotransmitter detection because they have higher aspect ratios enabling monoamine adsorption and lower limits of detection, faster electron transfer kinetics, and a resistance to surface fouling. Several methods to modify CFMEs with CNTs have resulted in increases in sensitivity, but have also increased noise and led to irreproducible results. In this study, we utilize commercially available CNT-yarns to make microelectrodes as enhanced neurotransmitter sensors for neurotransmitters such as serotonin. CNT-yarn microelectrodes have significantly higher sensitivities (peak oxidative currents of the cyclic voltammograms) than CFMEs and faster electron transfer kinetics as measured by peak separation (Delta(EP)) values. Moreover, both serotonin and dopamine are adsorption controlled to the surface of the electrode as measured by scan rate and concentration experiments. CNT yarn microelectrodes also resisted surface fouling of serotonin onto the surface of the electrode over thirty minutes and had a wave application frequency independent response to sensitivity at the surface of the electrode.