Large-scale detection and analysis of adenosine-to-inosine RNA editing during development in Plutella xylostella
Large-scale detection and analysis of adenosine-to-inosine RNA editing during development in Plutella xylostella
复制标题
小菜蛾发育过程中腺苷至肌苷 RNA 编辑的大规模检测和分析。
DOI：
10.1007/s00438-014-0968-4
复制
发表时间：
2015-06-01
影响因子：
3.1
通讯作者：
Li, Fei
中科院分区：
文献类型：
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作者：
He, Tao;Lei, Wenjie;Li, Fei
腺苷转肌苷（A-to-I）RNA编辑是RNA的位点特异性修饰，增加了转录组和蛋白质组的多样性。大多数昆虫经历完全变态，包括四个生命周期阶段：卵，幼虫，蛹和成虫。许多先前的研究已经证实，RNA编辑事件以发育特异性的方式发生;换句话说，RNA编辑水平在变态过程中发生变化。在这里，我们描述了一个努力，以确定RNA编辑事件的发展特异性，使用大规模的计算分析RNA-seq数据来自四个发育阶段的小菜蛾，小菜蛾。1187个A-to-IRNA编辑位点被预测为发育阶段特异性的（错误发现率< 0.01），其中1,094个位点位于蛋白质编码区。编辑152个位点导致改变的氨基酸残基。通过比较cDNA序列与小菜蛾生活史不同阶段的相应基因组位点，验证了一个假定的成虫特异性A至I RNA编辑位点。我们的研究结果将有助于阐明A-to-I RNA编辑在变态调节中的作用。需要进一步的研究详细说明编辑程度的变化，以确定迄今未知的调节因子如何参与编辑机制以及编辑的生物功能是什么。
Adenosine-to-inosine (A-to-I) RNA editing is site-specific modification of RNAs that increases the diversity of the transcriptome and proteome. Most insects undergo complete metamorphosis, including four life cycle stages: egg, larva, pupa and adult. Many previous studies have confirmed that RNA-editing events occur in a development-specific manner; in other words, RNA-editing levels change during metamorphosis. Here, we describe an effort to identify the developmental specificity of RNA-editing events using a large-scale computational analysis of RNA-seq data derived from four developmental stages of the diamondback moth, Plutella xylostella. One thousand one hundred and eighty-seven A-to-I RNA-editing sites were predicted to be developmental stage specific (false-discovery rate < 0.01) and 1,094 of these sites were located in protein-coding regions. Editing of 152 sites resulted in an altered amino acid residue. A putative adult-specific A-to-I RNA-editing site was verified by comparing cDNA sequences with its corresponding genomic locus at different stages of the P. xylostella life cycle. Our findings will help elucidate the role of A-to-I RNA editing in the regulation of metamorphosis. Further studies detailing changes in the extent of editing are needed to establish how as yet unknown regulatory factors are involved in the editing mechanism and what biological functions' editing serves.