Differential protein expression in the susceptible and resistant Myzus persicae (Sulzer) to imidacloprid
Differential protein expression in the susceptible and resistant Myzus persicae (Sulzer) to imidacloprid
复制标题
桃蚜（Sulzer）对吡虫啉敏感和抗性的差异蛋白表达
DOI：
10.1016/j.pestbp.2014.09.002
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发表时间：
2014-10-01
影响因子：
4.7
通讯作者：
Shang, ShengHua
中科院分区：
文献类型：
--
作者：
Meng, JianYu;Zhang, ChangYu;Shang, ShengHua
吡虫啉（IMI）对桃蚜产生抗药性，是一种严重的农业经济害虫，在世界范围内被广泛用于防治桃蚜。为了更好地了解桃蚜耐IMI的机制，我们进行了比较蛋白质组学分析。采用双向凝胶电泳法提取耐药株和敏感株的总蛋白。共检测到1300多个蛋白点，其中14个蛋白点较丰富，14个蛋白点较不丰富。质谱分析和数据库检索帮助我们鉴定了25种差异丰富的蛋白质。鉴定出的蛋白质可分为信号转导、RNA加工、蛋白质加工、转运加工、应激反应、代谢和细胞骨架结构等功能群。本研究首次分析了IMI易感和耐药桃蚜的差异表达蛋白，为探讨IMI耐药机制提供了新的思路。(C) 2014爱思唯尔公司版权所有。
Myzus persicae, a serious economic agricultural pest, has developed resistance to imidacloprid (IMI), which was widely used to control this aphid worldwide. To gain a better understanding of the mechanisms of IMI resistance in M. persicae, we carried out a comparative proteomic analysis. Total proteins of the IMI-susceptible and resistant strains were extracted and separated by two-dimensional gel electrophoresis. More than 1300 protein spots were reproducibly detected, including 14 that were more abundant and 14 less abundant. Mass spectrometry analysis and database searching helped us to identify 25 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, transport processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in IMI-susceptible and resistant M. Persicae, and gives new insights into the mechanisms of IMI resistance in M. persicae. (C) 2014 Elsevier Inc. All rights reserved.