Dorsal hump morphology in pink salmon (Oncorhynchus gorbuscha)
Dorsal hump morphology in pink salmon (Oncorhynchus gorbuscha)
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粉红鲑鱼（Oncorhynchus gorbuscha）的背驼峰形态
DOI：
10.1002/jmor.20234
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发表时间：
2014
影响因子：
1.5
通讯作者：
Kudo Hideaki
中科院分区：
文献类型：
--
作者：
Kanta susuki;Masaki Ichimura;Yosuke Koshino;Masehide Kaeriyama;Yasuyuki Takagi;Shinji Adechi;Kudo Hideaki
成熟的雄性太平洋鲑鱼（Oncorhynchus属）在产卵期间会发育一个背驼峰，作为一种第二性征。以前的大体解剖学研究表明，鲑鱼的背驼峰主要由软骨组织组成（Davidson [1935] J Morphol 57：169-183）。然而，这种驼峰的组织学和生物化学特征知之甚少。本研究利用组织化学技术和电泳技术对粉鲑背驼峰的显微结构和组成进行了详细的分析。在成熟的男性，游离的神经间棘和神经棘位于一条线附近的背驼峰的中间隔膜。在背驼峰内未检测到软骨组织。纤维和粘液结缔组织主要存在于背驼峰的三个区域：i）中间隔，ii）远端区域和iii）新月形区域。中间隔和远侧区均由含水量高的结缔组织组成，其中含有弹性纤维和透明质酸。结果还表明，与未成熟和成熟的雄性相比，成熟的雄性背驼峰结缔组织的脂质含量显着降低。虽然驼峰的新月形区域由结缔组织组成，但不含弹性纤维、透明质酸或脂质。超微结构观察发现，背驼峰内的结缔组织均由胶原纤维组成。从这些组织中提取的胶原蛋白的凝胶电泳发现，背驼峰中的胶原蛋白由I型胶原蛋白组成，就像鲑鱼皮中的情况一样。这些结果表明，在雄性粉红鲑鱼的背驼峰形成的结缔组织，而不是软骨的量的增加，和自由神经间棘和神经棘的生长的结果。J. Morphol. 275：514-527，2014.© 2013 Wiley Periodicals，Inc.
Mature male Pacific salmon (GenusOncorhynchus) develop a dorsal hump, as a secondary male sexual characteristic, during the spawning period. Previous gross anatomical studies have indicated that the dorsal humps of salmon are mainly composed of cartilaginous tissue (Davidson [1935] J Morphol 57:169–183.) However, the histological and biochemical characteristics of such humps are poorly understood. In this study, the detailed microstructures and components of the dorsal humps of pink salmon were analyzed using histochemical techniques and electrophoresis. In mature males, free interneural spines and neural spines were located in a line near to the median septum of the dorsal hump. No cartilaginous tissue was detected within the dorsal hump. Fibrous and mucous connective tissues were mainly found in three regions of the dorsal hump: i) the median septum, ii) the distal region, and iii) the crescent‐shaped region. Both the median septum and distal region consisted of connective tissue with a high water content, which contained elastic fibers and hyaluronic acid. It was also demonstrated that the lipid content of the dorsal hump connective tissue was markedly decreased in the mature males compared with the immature and maturing males. Although, the crescent‐shaped region of the hump consisted of connective tissue, it did not contain elastic fibers, hyaluronic acid, or lipids. In an ultrastructural examination, it was found that all of the connective tissues in the dorsal hump were composed of collagen fibers. Gel electrophoresis of collagen extracts from these tissues found that the collagen in the dorsal hump is composed of Type I collagen, as is the case in salmon skin. These results indicate that in male pink salmon the dorsal hump is formed as a result of an increase in the amount of connective tissue, rather than cartilage, and the growth of free interneural spines and neural spines. J. Morphol. 275:514–527, 2014. © 2013 Wiley Periodicals, Inc.