Comparative differences in the composition and biomechanical properties of tibiae of seven- and seventy-two-week-old male and female broiler breeder chickens
Comparative differences in the composition and biomechanical properties of tibiae of seven- and seventy-two-week-old male and female broiler breeder chickens
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DOI：
10.1093/ps/78.8.1232
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发表时间：
1999-08-01
期刊：
影响因子：
4.4
通讯作者：
Tierce, JF
中科院分区：
文献类型：
--
作者：
Rath, NC;Balog, JM;Tierce, JF
骨骼问题在年轻和年老的家禽中都很常见，并且通常与骨质疏松有关。它们影响农场的死亡率和加工厂的谴责，从而引起福利和经济问题。为了了解骨强度的基础，对 7 周龄和 72 周龄雄性和雌性肉种鸡胫骨的干骺端组织学、组成和生物力学特性进行了比较。生化成分包括灰分、胶原蛋白、蛋白聚糖、转化生长因子-β (TGF-β)、吡啶交联和有机基质相关荧光。测量血液变量以确定这些鸟类与骨骼生理学相关的代谢状态。虽然7周龄雄性和雌性的血液化学没有差异，但幼鸟和老鸟之间存在一些差异。老年女性的钙、甘油三酯和铁的水平高于老年男性。 72周龄母鸡胶原蛋白含量减少，蛋白多糖含量增加。 7周龄雌性鸟类骨骼中TGF-β含量高于其他群体的鸟类。分别使用断裂载荷和杨氏模量测量的骨强度和刚度在年老的鸟类中较高。 72周龄母鸡中髓质骨的存在并不影响它们的骨强度，尽管它降低了应变值并增加了杨氏模量。与其他组相比，72周龄母鸡的无机基质含量较高。老鸟的有机基质中羟赖氨酰吡啶啉（HP）和赖氨酰吡啶啉（LP）的水平以及胶原酶可提取的荧光水平较高。年长鸟类的脱钙骨基质也比年轻鸟类对细菌胶原酶更敏感。骨强度与其灰分含量、密度、吡啶交联和基质的荧光呈正相关。然而，吡啶鎓交联和有机基质的荧光之间的相关性很强。这些结果表明骨强度受其胶原交联含量的影响。
Skeletal problems are common in both young and old poultry and are often related to bone weakness. They affect mortality on the farm and condemnations within processing plant and thus raise both welfare and economic concerns. To understand the basis of bone strength, the metaphyseal histology, composition, and the biomechanical properties of tibiae from 7- and 72-wk-old male and female broiler breeder chickens were compared. The biochemical constituents included ash, collagen, proteoglycan, transforming growth factor-beta (TGF-beta), pyridinium crosslinks, and the organic matrix-associated fluorescence. Blood variables were measured to determine the metabolic status of these birds as related to bone physiology. Although there were no differences in blood chemistry of 7-wk-old males and females, there were several differences between young and old birds. The levels of calcium, triglyceride, and iron were higher in older females than in older males. The collagen content was reduced, and the proteoglycan content increased in 72-wk-old hens. The TGF-beta content of bones from 7-wk-old females was higher than that of other groups of birds. Bone strength and stiffness, measured using loads at break and Young's modulus, respectively, were higher in older birds. The presence of medullary bones in 72-wk-old hens did not affect their bone strength, although it reduced strain values and increased Young's modulus. Compared to other groups, the 72-wk-old hens had a higher content of an inorganic matrix. The levels of hydroxylysylpyridinoline (HP) and lysyl-pyridinoline (LP) and the collagenase-extractable fluorescence of the organic matrix from older birds was higher. The decalcified bone matrix from older birds also showed higher susceptibility to bacterial collagenase than their younger counterparts. Bone strength showed positive correlations with its ash content, density, pyridinium crosslinks, and the fluorescence of the matrix. However, the correlation was strong with both pyridinium crosslinks and the fluorescence of the organic matrix. These results suggest that bone strength is influenced by the content of its collagen crosslinks.