Capillary electrophoresis and indirect UV detection as a fast and simple analytical tool for bacterial taxonomy
Capillary electrophoresis and indirect UV detection as a fast and simple analytical tool for bacterial taxonomy
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DOI：
10.1016/s0021-9673(97)00310-5
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发表时间：
1997-09-26
影响因子：
4.1
通讯作者：
Puig, P
中科院分区：
文献类型：
--
作者：
Arellano, M;ElKaddouri, S;Puig, P
短链甲基化脂肪酸由于其高挥发性而难以使用常规技术如气相色谱法进行研究。此外，样品的处理是长期和挑剔的。脂肪酸是表征厌氧菌的非常有趣的物质。本工作建立了一种快速、简便的毛细管电泳-间接紫外检测法分析不同短链脂肪酸的新方法。我们分析了不同厌氧细菌的培养基，发现了一些浓度模式，在物种水平上的判别。在分析之前，将培养基简单过滤并稀释。(C)1997年Elsevier Science B.V.
Short-chain methylated fatty acids are difficult to study using conventional techniques such as gas chromatography, because of their high volatility. Moreover, the treatment of the samples is long and fastidious. Fatty acids are very interesting substances for the characterization of anaerobic bacteria. In this work we develop a new fast and simple method using capillary electrophoresis and indirect UV detection for the analysis of different short-chain fatty acids. We analyzed culture media of different anaerobic bacteria and found some concentration patterns which are discriminant at the species level. Prior to analysis the culture media were simply filtrated and diluted. (C) 1997 Elsevier Science B.V.