Characterization of a pattern recognition molecule vitellogenin from carp (Cyprinus carpio)
Characterization of a pattern recognition molecule vitellogenin from carp (Cyprinus carpio)
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鲤鱼 (Cyprinus carpio) 模式识别分子卵黄蛋白原的表征。
DOI：
10.1016/j.imbio.2008.10.003
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发表时间：
2009-04-01
期刊：
影响因子：
2.8
通讯作者：
Gao, Chun-Ren
中科院分区：
文献类型：
--
作者：
Liu, Qing-Hui;Zhang, Shi-Cui;Gao, Chun-Ren
模式识别蛋白通过与入侵病原体表面的分子结合并启动宿主防御反应而在先天免疫应答中发挥作用。为探讨卵黄蛋白原（Vg）在鱼类天然免疫中的作用，采用凝胶过滤结合二乙氨基乙基（DEAE）层析的方法从鲤鱼中分离纯化了卵黄蛋白原（Vg）。纯化后的Vg经MALDI-TOF质谱确证。抗菌活性分析表明，Vg对大肠杆菌和金黄色葡萄球菌的抑菌活性呈剂量依赖性抑制。Vg与革兰氏阴性菌和革兰氏阳性菌的表面结合。它还能凝集E. coli和革兰氏阳性菌S.而对酿酒酵母的抑制作用较弱。Vg对革兰氏阴性菌脂多糖具有较强的结合活性。Vg处理的巨噬细胞对E. coli和革兰氏阳性菌S.金黄色。Vg还与巨噬细胞结合，作为调理素起促进吞噬作用作用。结果表明，Vg在鱼类的抗菌防御中起着模式识别分子和调理素的作用，在鱼类的先天免疫中起着效应子的作用。(C)2008爱思唯尔有限公司All rights reserved.
Pattern recognition proteins function in innate immune responses by binding to molecules on the surface of invading pathogens and initiating host defense reactions. To explore the role of vitellogenin (Vg) in fish innate immunity, we purified Vg from Carp by gel filtration combined with diethylaminoethyl (DEAE) chromatography. The purified Vg was confirmed by MALDI-TOF mass spectrometry. Antibacterial activity analysis showed that Vg inhibited bacterial activity to Escherichia coli and Staphylococcus aureus in a dose-dependent manner. Vg bound to the surface of Gram-negative and Gram-positive bacteria. It also agglutinated E. coli and S. aureus and weakly to Saccharomyces cerevisiae. Vg showed a strong binding activity to lipopolysaccharides from Gram-negative bacteria. Vg-treated macrophage enhanced phagocytosis to E. coli and S. aureus. Vg also bind with macrophage function as opsonins to promote phagocytosis. The results suggest that Vg serves as a pattern recognition molecule and opsonins in antibacterial defense and as an effector in fish innate immunity. (C) 2008 Elsevier GmbH. All rights reserved.