Induction of high endothelial venule-like vessels expressing GlcNAc6ST-1-mediated L-selectin ligand carbohydrate and mucosal addressin cell adhesion molecule 1(MAdCAM-1) in a mouse model of" Candidatus Helicobacter heilmannii"-induced gastritis and gastri
Induction of high endothelial venule-like vessels expressing GlcNAc6ST-1-mediated L-selectin ligand carbohydrate and mucosal addressin cell adhesion molecule 1(MAdCAM-1) in a mouse model of" Candidatus Helicobacter heilmannii"-induced gastritis and gastri
复制标题
在“海尔曼螺杆菌”诱导的胃炎和胃炎小鼠模型中诱导表达 GlcNAc6ST-1 介导的 L-选择素配体碳水化合物和粘膜寻址素细胞粘附分子 1（MAdCAM-1）的高内皮小静脉样血管
DOI：
10.1111/j.1523-5378.2010.00801.x
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发表时间：
2010
期刊：
影响因子：
4.4
通讯作者：
Ota H
中科院分区：
文献类型：
--
作者：
Suzuki A;Kobayashi M;Matsuda K;Matsumoto T;Kawakubo M;Kumazawa S;Koide N;Miyagawa S;Ota H
背景：海氏螺杆菌（Helicobacter heilmannii）引起慢性胃炎，最终导致胃粘膜相关淋巴组织（MALT）B细胞型淋巴瘤。本研究是使用动物模型的感染与“EscheridatusHelicobacterheilmannii”，以阐明这种慢性炎症是如何诱导或maintained.Materials和方法：BALB/c小鼠感染的“EscheridatusHelicobacterheilmannii”分离株SH 4。在感染后8、26、54和83周检查动物。 切除动物的胃，并对外周淋巴结地址素（PNAd）和粘膜地址素细胞粘附分子1（MAdCAM-1）、“幽门螺杆菌”和CD 45 R/B220进行免疫染色。进行了L-和E-选择素·IgM嵌合蛋白的体外结合试验。采用真实的实时荧光定量聚合酶链反应（Real-time PCR，RT-PCR）技术检测N-乙酰葡萄糖胺-6-O-磺基转移酶（GlcNAc 6STs）基因的转录水平。在感染后54周和83周，一些动物发生了B-细胞型MALT淋巴瘤。在发生慢性胃炎和MALT淋巴瘤的感染动物中诱导表达PNAd和MAdCAM-1的高内皮微静脉（HEV）样血管。随着慢性炎症的进展，HEV样血管的数量增加。诱导的HEV样血管被L-和E-选择素·IgM嵌合蛋白结合。结论：表达GlcNAc 6ST-1介导的L-选择素配体碳水化合物和MAdCAM-1的HEV样血管可能在海氏螺杆菌（Helicobacter heilmannii）诱导的慢性胃炎和MALT淋巴瘤的发病机制中起重要作用。
Background:“CandidatusHelicobacter heilmannii” induce chronic gastritis, which eventually leads to gastric B‐cell type mucosa‐associated lymphoid tissue (MALT) lymphoma. This study was performed using an animal model of infection with “CandidatusHelicobacter heilmannii” to elucidate how this chronic inflammation is induced or maintained.Materials and Methods:BALB/c mice were infected with the “CandidatusHelicobacter heilmannii” isolate SH4. The animals were examined at 8, 26, 54, and 83 weeks after the infection. The stomach of the animals was resected and immunostained for peripheral lymph node addressin (PNAd) and mucosal addressin cell adhesion molecule 1 (MAdCAM‐1), “CandidatusHelicobacter heilmannii,” and CD45R/B220. An in vitro binding assay with L‐ and E‐selectin·IgM chimeric proteins was performed. Real‐time polymerase chain reaction was used to evaluate transcripts ofN‐acetylglucosamine‐6‐O‐sulfotransferases (GlcNAc6STs), which direct the expression of the PNAd and MAdCAM‐1.Results:Chronic gastritis developed in the infected animals, and its severity increased with the duration of the infection. B‐cell type MALT lymphoma developed in some animals at 54 and 83 weeks after infection. PNAd‐ and MAdCAM‐1‐expressing high endothelial venule (HEV)‐like vessels were induced in infected animals which developed chronic gastritis and MALT lymphoma. The number of HEV‐like vessels increased as chronic inflammation progressed. The induced HEV‐like vessels were bound by L‐ and E‐selectin·IgM chimeric protein. mRNA expressions of GlcNAc6ST‐1 and MAdCAM‐1 increased in the infected animals.Conclusions:HEV‐like vessels expressing GlcNAc6ST‐1‐mediated L‐selectin ligand carbohydrate and MAdCAM‐1 may play a crucial role in the pathogenesis of “CandidatusHelicobacter heilmannii”‐induced chronic gastritis and MALT lymphoma.