BASIC FIBROBLAST GROWTH-FACTOR INDUCES 3T3 FIBROBLASTS TO SYNTHESIZE AND SECRETE A CYCLOPHILIN-LIKE PROTEIN AND BETA-2-MICROGLOBULIN
BASIC FIBROBLAST GROWTH-FACTOR INDUCES 3T3 FIBROBLASTS TO SYNTHESIZE AND SECRETE A CYCLOPHILIN-LIKE PROTEIN AND BETA-2-MICROGLOBULIN
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DOI：
10.1016/0167-4889(91)90077-b
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发表时间：
1991-10-26
期刊：
影响因子：
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通讯作者：
NILSENHAMILTON, M
中科院分区：
文献类型：
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作者：
DAVIS, TR;TABATABAI, L;NILSENHAMILTON, M
当受到成纤维细胞生长因子（FGF）刺激时，BALB / c 3 T3细胞合成并分泌大量的五种蛋白质，称为“超诱导蛋白”或SIPs。 如果细胞在诱导期间用环己酰亚胺处理，这些蛋白质的表达会大大增强。 分子量为24 kDa的蛋白质（SIP 24）已被纯化并制备了抗血清，该蛋白质是N-糖基化的，可能在结构上受到一个或多个分子内二硫键的限制。 四种肽中的三种的氨基酸序列显示出与亲环素的显著同一性，亲环素是一种被认为介导环孢菌素A的免疫抑制作用的丰富的细胞质蛋白。 亲环素家族的几个成员已被确定，并已报道了两个亲环素样蛋白的cDNA克隆与信号序列。 在这里，我们表明，至少有一个亲环蛋白样蛋白的分泌，其表达受生长因子。 发现12.5 kDa蛋白质（SIP12.5）被抗人β-2-微球蛋白的抗血清免疫沉淀。 这种蛋白质被干扰素强烈诱导，干扰素是β-2-微球蛋白基因的特征。 因此，FGF刺激小鼠胚胎3 T3细胞产生两种与免疫调节分子相关的蛋白质。 这可能反映了免疫细胞和非免疫细胞之间的相互作用，这种相互作用发生在体内过程中，例如当生长因子局部释放时伤口愈合。
When stimulated by fibroblast growth factor (FGF) BALB / c 3T3 cells synthesize and secrete elevated amounts of five proteins called the 'superinducible proteins', or SIPs. The expression of these proteins is greatly enhanced if the cells are treated with cycloheximide during induction. The 24 kDa protein (SIP24) has been purified and antiserum raised against it. This protein is N-glycosylated and probably structurally constrained by one or more intramolecular disulfide bonds. The amino acid sequences of three of four peptides show significant identity with cyclophilin, an abundant cytoplasmic protein believed to mediate the immunosuppressive effects of cyclosporin A. Several members of the cyclophilin family have been identified, and cDNA clones of two cyclophilin-like proteins with signal sequences have been reported. Here we show that at least one cyclophilin-like protein is secreted and that its expression is regulated by growth factors. The 12.5 kDa protein (SIP12.5) was found to be immunoprecipitated by an antiserum raised to human beta-2-microglobulin. This protein is strongly induced by interferon, which is a characteristic of the beta-2-microglobulin gene. Thus, FGF stimulates mouse embryo 3T3 cells to produce two proteins related to immune regulatory molecules. This may reflect an interaction between immune cells and nonimmune cells that occurs in vivo during processes such as wound healing when growth factors are released locally.