cDNA cloning, expression analysis, and mapping of the mouse Nedd4 gene
cDNA cloning, expression analysis, and mapping of the mouse Nedd4 gene
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DOI：
10.1006/geno.1996.4582
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发表时间：
1997-03-15
期刊：
影响因子：
4.4
通讯作者：
Jenkins, NA
中科院分区：
文献类型：
--
作者：
Kumar, S;Harvey, KF;Jenkins, NA
Nedd4基因最初通过消减克隆方法被鉴定为在小鼠胚胎脑中高表达的转录本。Nedd4基因的克隆表明，Nedd4编码一个约103 kDa的蛋白质，由一个钙离子和磷脂结合结构域、三个可能的蛋白质-蛋白质相互作用结构域(WW结构域)和一个类似泛素-蛋白质连接酶结构域的羧基末端区域(HECT结构域)组成。在小鼠胚胎中，Nedd4在中枢神经系统中的表达在神经发生过程中最高，并随着发育的进展而下降。除了中枢神经系统外，Nedd4在各种胚胎组织中都有表达，并在大多数成年组织中持续存在。使用针对融合蛋白的抗体，我们证明Nedd4蛋白定位于细胞质。我们使用种间回交板将小鼠Nedd4基因定位在9号染色体上。Nedd4映射到先前定义的人类和小鼠染色体之间的同源区域，从而提供了关于物种间比较图谱的额外信息。(C)1997年学术出版社。
The Nedd4 gene was initially identified by a subtraction cloning approach as a highly expressed transcript in the mouse embryonic brain. Cloning of the Nedd4 cDNA indicated that it can encode a protein of approximately 103 kDa, consisting of a Ca2+ and phospholipid binding domain, three putative protein-protein interaction domains (the WW domains), and a carboxyl-terminus region similar to the ubiquitin-protein ligase domain (hect domain). In mouse embryos, the expression of Nedd4 in the central nervous system is highest during neurogenesis and decreases as development progresses. In addition to the central nervous system, the expression of Nedd4 is detected in various embryonic tissues and persists in most adult tissues. Using an antibody raised against a fusion protein, we show that Nedd4 protein is localized to the cellular cytoplasm. We have mapped the mouse Nedd4 gene to chromosome 9 using an interspecific backcross panel. Nedd4 maps to a previously defined homologous region between human and mouse chromosomes and thus provides additional information regarding interspecies comparative mapping. (C) 1997 Academic Press.