Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa.
Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa.
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DOI：
10.1371/journal.pone.0279437
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Petersen-Jones, Simon M.
中科院分区：
文献类型：
--
作者：
Occelli, Laurence M.;Sun, Kelian;Winkler, Paige A.;Morgan, Brandy J.;Petersen-Jones, Simon M.
研究视网膜环磷酸鸟苷（cGMP）水平升高是否反映在PDE 6A-/-犬的血浆水平中。从2月龄野生型狗（PDE 6A +/+，N = 6）、杂合狗（PDE 6A +/-，N = 4）和患病狗（PDE 6A-/-，N = 3）收集视网膜，从2月龄野生型狗（PDE 6A +/+，N = 5）、杂合狗（PDE 6A +/-，N = 5）和患病狗（PDE 6A-/-，N = 5）收集血浆。通过ELISA测量视网膜和血浆样品。2月龄时PDE 6A-/-狗视网膜样品中的cGMP水平显著升高。野生型和PDE 6A-/-或PDE 6A +/-幼犬之间的血浆cGMP水平无显著差异。然而，PDE 6A-/-幼犬的血浆cGMP水平显著低于PDE 6A +/-幼犬。与对照犬相比，PDE 6A-/-犬血浆中的cGMP水平未升高。在2个月时间点，cGMP血浆水平不是疾病的有用生物标志物。
To investigate whether raised levels of retinal cyclic guanosine monophosphate (cGMP) was reflected in plasma levels in PDE6A-/- dogs. Retina was collected from 2-month-old wildtype dogs (PDE6A+/+, N = 6), heterozygous dogs (PDE6A+/-, N = 4) and affected dogs (PDE6A-/-, N = 3) and plasma was collected from 2-month-old wildtype dogs (PDE6A+/+, N = 5), heterozygous dogs (PDE6A+/-, N = 5) and affected dogs (PDE6A-/-, N = 5). Retina and plasma samples were measured by ELISA. cGMP levels in retinal samples of PDE6A-/- dogs at 2 months of age were significantly elevated. There was no significant difference in plasma cGMP levels between wildtype and PDE6A-/- or PDE6A+/- puppies. However, the plasma cGMP levels of the PDE6A-/- puppies were significantly lower than that of PDE6A+/- puppies. cGMP levels in the plasma from PDE6A-/- was not elevated when compared to control dogs. At the 2-month timepoint, cGMP plasma levels would not be a useful biomarker for disease.
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