PAIRED ARRANGEMENT OF NONHOMOLOGOUS CENTROMERES DURING VERTEBRATE SPERMIOGENESIS
PAIRED ARRANGEMENT OF NONHOMOLOGOUS CENTROMERES DURING VERTEBRATE SPERMIOGENESIS
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DOI：
10.1016/0014-4827(90)90130-3
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发表时间：
1990-03-01
影响因子：
3.7
通讯作者：
SCHMID, M
中科院分区：
文献类型：
--
作者：
HAAF, T;GRUNENBERG, H;SCHMID, M
使用人抗着丝粒抗体的间接免疫荧光染色来研究脊椎动物精子发生过程中着丝粒的位置。许多两栖动物物种的染色体数量较少，但精子细胞和精子非常大。动粒点的数量与单倍体染色体的数量完全相关。这意味着动粒复制发生在减数分裂 I 和减数分裂 II 之间的间隔内。非同源着丝粒在精子发生的整个过程和成熟精子中串联排列。在无尾藻和有尾藻的生精细胞中发现了更高阶的着丝粒排列。在哺乳动物中，由于精子发生过程中染色质的极度浓缩和染色体数量较多，免疫荧光分析变得复杂。然而，在哺乳动物圆形精子细胞中观察到着丝粒-着丝粒关联，并且在睾丸精子中偶尔观察到着丝粒-着丝粒关联。这表明着丝粒成对关联是减数分裂后着丝粒排列的普遍原则。
Indirect immunofluorescence staining with human anti-kinetochore antibodies was used to study the position of centromeres during vertebrate spermiogenesis. Many species of Amphibia have a low chromosomes number and very large spermatids and spermatozoa. The number of kinetochore dots correlates exactly with the haploid chromosome number. This implies that kinetochore duplication occurs in the interval between meiosis I and meiosis II. The nonhomologous centromeres are arranged in tandem during the entire course of spermiogenesis and in mature spermatozoa. A higher order centromere arrangement was found in spermiogenic cells of Anura and Urodela. In mammals, immunfluorescence analysis is complicated by the extreme condensation of chromatin during spermiogenesis and the high chromosome numbers. Nevertheless, centromere-centromere associations were observed in mammalian round spermatids and sporadically in testicular spermatozoa. This indicates that pair-wise association of centromeres is a universal principle of centromere arrangement at the postmeiotic stage.