Immunity to onchocerciasis: cells from putatively immune individuals produce enhanced levels of interleukin-5, gamma interferon, and granulocyte-macrophage colony-stimulating factor in response to Onchocerca volvulus larval and male worm antigens.
Immunity to onchocerciasis: cells from putatively immune individuals produce enhanced levels of interleukin-5, gamma interferon, and granulocyte-macrophage colony-stimulating factor in response to Onchocerca volvulus larval and male worm antigens.
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对盘尾丝虫病的免疫力：来自推定免疫个体的细胞会产生较高水平的白介素 5、γ 干扰素和粒细胞巨噬细胞集落刺激因子，以响应盘尾丝虫幼虫和雄性蠕虫抗原。
DOI：
10.1128/iai.68.4.1905-1911.2000
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发表时间：
2000
影响因子：
3.1
通讯作者：
Lustigman,S
中科院分区：
文献类型：
--
作者：
Turaga,PS;Tierney,TJ;Bennett,KE;McCarthy,MC;Simonek,SC;Enyong,PA;Moukatte,DW;Lustigman,S
研究了喀麦隆盘尾丝虫病高流行区个体的抗原特异性白细胞介素-5 （IL-5）、γ干扰素（IFN-γ）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）反应。将长尾丝虫第三期幼虫（L3）、蜕皮期L3 （mL3）和成虫雄尾丝虫粗提物（M-OvAg）对抗原的反应与成虫雌虫和皮肤微丝虫对抗原的反应进行了比较。比较推定免疫个体（PI）和感染个体（INF）的细胞因子反应。在这些个体中发现了不同的细胞因子IL-5 （Th2表型）和IFN-γ （Th1表型）对抗原的反应。在PI和INF中，Th2对所有抗原的反应都是显性的。然而，在整个PI组中，对幼虫抗原和成年雄性和成年雌性抗原的Th2反应增强，并且在PI的一个亚群中（27%至54.5%）对L3， mL3和M-OvAg抗原存在Th1反应。虽然PI对L3、mL3和M-OvAg抗原产生的GM-CSF水平明显高于INF，但两组对其他抗原的GM-CSF反应没有差异。本研究表明，与INF相比，PI具有不同的幼虫特异性和成年雄性特异性细胞因子反应，从而支持了PI免疫学研究将导致识别免疫机制和在保护性免疫中起作用的靶基因的前提。
Antigen-specific interleukin-5 (IL-5), gamma interferon (IFN-γ), and granulocyte-macrophage colony-stimulating factor (GM-CSF) responses in individuals living in an area of hyperendemicity for onchocerciasis in Cameroon were examined. The responses against antigens prepared fromOnchocerca volvulusthird-stage larvae (L3), molting L3 (mL3), and crude extract from adult males (M-OvAg) were compared to the responses against antigens from adult female worms and skin microfilariae. Cytokine responses for the putatively immune individuals (PI) and the infected individuals (INF) were compared. A differential cytokine profile of IL-5 (Th2 phenotype) and IFN-γ (Th1 phenotype) was found in these individuals in response to the antigens. In both the PI and the INF, Th2 responses against all the antigens tested were dominant. However, in the PI group as a whole, there was an enhanced Th2 response against the larval antigens and the adult male and adult female antigens, and a Th1 response in a subgroup of the PI (27 to 54.5%) against L3, mL3, and M-OvAg antigens was present. While the PI produced significantly higher levels of GM-CSF against L3, mL3, and M-OvAg antigens than the INF, there was no difference in the GM-CSF responses of the groups against the other antigens. The present study indicated that, in comparison to the INF, the PI have distinct larva-specific and adult male-specific cytokine responses, thus supporting the premise that immunological studies of the PI would lead to the identification of immune mechanisms and the target genes that play a role in protective immunity.