Analysis of rat heart in vivo by phosphorus nuclear magnetic resonance.
Analysis of rat heart in vivo by phosphorus nuclear magnetic resonance.
复制标题
磷核磁共振对大鼠体内心脏的分析。
DOI：
--
复制
发表时间：
1980
影响因子：
11.1
通讯作者：
P. Bore
中科院分区：
文献类型：
--
作者：
T. Grove;J. Ackerman;G. Radda;P. Bore
本文报道了73.83MHz高分辨~（31）P在体大鼠心脏的核磁共振谱。在活体大鼠中，可以观察到ATP、磷酸肌酸和Pi的心脏含量。在光谱中仅观察到少量全血2，3-二磷酸甘油酸，排除了血磷酸盐化合物掩盖心脏磷酸盐化合物光谱的可能性。在体内和灌流大鼠心脏的31 P核磁共振谱是相似的，并支持利用灌流大鼠心脏作为模型系统，用于研究在体内心脏的高能磷酸盐代谢。高能磷酸化合物的动态流量进行了研究，通过使大鼠呼吸停止。在这个实验中，心脏遵循心脏骤停时已知的经典代谢模式;磷酸肌酸和ATP的浓度下降，而Pi的浓度增加。在体大鼠心脏~（31）P核磁共振分析是研究心脏高能磷酸盐代谢的一种实用可行的方法。
High-resolution 31P nuclear magnetic resonance spectra at 73.83 MHz are reported for rat heart in vivo. In live rats, it was possible to observe the cardiac content of ATP, phosphocreatine, and Pi. Only a small amount of whole-blood 2,3-diphosphoglycerate was observed in the spectra, precluding the possibility that blood phosphate compounds were masking the spectra of cardiac phosphate compounds. The 31P nuclear magnetic resonance spectra of in vivo and perfused rat hearts were similar and support the utilization of the perfused rat heart as a model system for studying high-energy phosphate metabolism of the heart in vivo. The dynamic flux of high-energy phosphate compounds was investigated by subjecting the rat to respiratory arrest. In this experiment, the heart followed the classic metabolic pattern known to occur during cardiac arrest; phosphocreatine and then ATP decreased in concentration while Pi increased in concentration. The 31P nuclear magnetic resonance analysis of rat heart in vivo is demonstrated to be a practical and feasible method for studying cardiac high-energy phosphate metabolism.