Photoacoustic microscopy of microvascular responses to cortical electrical stimulation
Photoacoustic microscopy of microvascular responses to cortical electrical stimulation
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DOI：
10.1117/1.3594785
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发表时间：
2011-07-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Tsytsarev, Vassiliy;Hu, Song;Wang, Lihong V.
皮质血流动力学功能成像的进步极大地促进了对神经血管耦合的理解。在这项研究中，使用无标记光学分辨率光声显微镜（OR-PAM）来监测微血管对通过颅口直接电刺激小鼠体感觉皮层的反应。反应表现为两种形式：血管收缩和血管扩张。通过改变刺激强度，在单个血管中观察到这两种反应形式之间的转变。从同一母血管分叉的两个子血管的电流依赖性反应之间发现了显著的相关性。对三种不同动物的27条血管进行统计分析，进一步表征了微血管反应的时空特征和当前依赖性。我们的研究结果表明，OR-PAM是在微观水平上研究神经血管耦合的有价值的工具。(C) 2011光学仪器工程师学会（SPIE）(DOI: 10.1117/1.3594785)
Advances in the functional imaging of cortical hemodynamics have greatly facilitated the understanding of neurovascular coupling. In this study, label-free optical-resolution photoacoustic microscopy (OR-PAM) was used to monitor microvascular responses to direct electrical stimulations of the mouse somatosensory cortex through a cranial opening. The responses appeared in two forms: vasoconstriction and vasodilatation. The transition between these two forms of response was observed in single vessels by varying the stimulation intensity. Marked correlation was found between the current-dependent responses of two daughter vessels bifurcating from the same parent vessel. Statistical analysis of twenty-seven vessels from three different animals further characterized the spatial-temporal features and the current dependence of the microvascular response. Our results demonstrate that OR-PAM is a valuable tool to study neurovascular coupling at the microscopic level. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI:10.1117/1.3594785]