Highly fluorescent nitrogen-doped carbon dots derived from Phyllanthus acidus utilized as a fluorescent probe for label-free selective detection of Fe3+ ions, live cell imaging and fluorescent ink
Highly fluorescent nitrogen-doped carbon dots derived from Phyllanthus acidus utilized as a fluorescent probe for label-free selective detection of Fe3+ ions, live cell imaging and fluorescent ink
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DOI：
10.1016/j.bios.2017.07.076
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发表时间：
2018-01-15
影响因子：
12.6
通讯作者：
Lee, Yong Rok
中科院分区：
文献类型：
--
作者：
Atchudan, Raji;Edison, Thomas Nesakumar Jebakumar Immanuel;Lee, Yong Rok
以叶下珠为碳源，氨水为氮源，采用简便、经济、一步水热法合成了高耐久荧光掺氮碳点。合成的FNCDs的平均尺寸为4.5+/-1 nm，在激发波长为365 inN的紫外光照射下，发出明亮的蓝色荧光。当激发波长为350 nm时，其量子产率为14%，最大发射波长为420 nm。X射线光电子能谱(XPS)和傅里叶变换红外光谱(FTIR)表征表明，FNCDs的形成主要由氮基和羟基组成，可以提供更多的吸附中心。此外，上述研究还揭示了FNCDs中氮与碳(C-N)的成功键合。所合成的高QY的FNCDs可作为检测Fe3+的有效荧光探针。基于归一化荧光强度与Fe3+离子浓度之间的线性关系，所制备的FNCDs可用于2-25微米宽浓度范围内Fe3+离子的无标记灵敏和选择性检测，检测下限为0.9微米。本研究证明所合成的FNCDs具有持久的荧光，很好地溶解于水中，因此在无标记灵敏和选择性检测Fe3+离子、荧光墨水和细胞成像等领域具有良好的生物兼容性和低细胞密度。
A facile, economical and one-step hydrothermal method is used to synthesize highly durable fluorescent nitrogen-doped carbon dots (FNCDs) by utilizing Phyllanthus acidus (P. acidus) and aqueous ammonia as the carbon and nitrogen sources, respectively. The synthesized FNCDs have an average size of 4.5 +/- 1 nm and showed bright blue fluorescence under the irradiation of UV-light at an excitation wavelength of 365 inn. It exhibits a quantum yield (QY) of 14% at an excitation wavelength of 350 nm with maximum emission at 420 nm. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy characterizations clearly showed the formation of FNCDs that predominantly consists of nitrogen and hydroxyl groups which can provide more adsorption sites. In addition, the above study reveals the successful bonding of nitrogen with carbon (C-N) in the FNCDs. The synthesized FNCDs with high QY can be used as efficient fluorescent probes for the detection of Fe3+. Based on the linear relationship between normalized fluorescence intensity and concentration of Fe3+ ions, the prepared FNCDs can be used for label-free sensitive and selective detection of Fe3+ ions in a wide concentration range of 2-25 mu M with a detection limit of 0.9 mu M. The present study proves that synthesized FNCDs has durable fluorescence, soluble in water very well and thus act as a promising candidate for the diverse applications such as label-free sensitive and selective detection of Fe3+, fluorescent ink and cellular imaging with good biocompatibility and low cytotmdcity.