HiChIP: efficient and sensitive analysis of protein-directed genome architecture
HiChIP: efficient and sensitive analysis of protein-directed genome architecture
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DOI：
10.1038/nmeth.3999
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发表时间：
2016-11-01
期刊：
影响因子：
48
通讯作者：
Chang, Howard Y.
中科院分区：
文献类型：
--
作者：
Mumbach, Maxwell R.;Rubin, Adam J.;Chang, Howard Y.
基因组构象是基因控制的核心，但却具有挑战性。在这里，我们提出了HiChIP，一种蛋白质中心染色质构象方法。与china - pet相比，HiChIP将构象信息读取的产量提高了10倍以上，并将输入要求降低了100倍以上。黏结蛋白的HiChIP显示了多尺度基因组结构，比原位Hi-C具有更高的信号背景比。
Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.