Histopathological effect of botulinum C2 toxin on mouse intestines
Histopathological effect of botulinum C2 toxin on mouse intestines
复制标题
肉毒杆菌C2毒素对小鼠肠道的组织病理学影响
作者：
I. Ohishi;Y. Odagiri
肉毒杆菌C2毒素在小鼠肠中具有组织病理学活性，并诱导肠袢中的液体积聚。毒素引起肠粘膜的变性和坏死变化：上皮细胞的细胞内空泡化，绒毛上皮的脱落和坏死，细胞间水肿，以及淋巴细胞和组织细胞的浸润。肠粘膜形态的可检测变化先于肠袢中液体积聚的增加。肠腔内注射肉毒杆菌C2毒素也诱导血浆蛋白渗漏到肠腔中，如通过伊文思蓝外渗所确定的。与肉毒杆菌C2毒素相反，霍乱和产气荚膜梭菌肠毒素对照引起非常轻微的蛋白质泄漏，尽管这些毒素诱导肠袢中显著的液体积聚。结果表明，肉毒杆菌C2毒素引起分泌反应的作用方式与霍乱和C.并且表明肉毒杆菌C2毒素通过对肠上皮细胞的细胞病变效应诱导分泌应答。
Botulinum C2 toxin has histopathological activity in the mouse intestine and induces fluid accumulation in intestinal loops. The toxin caused degenerative and necrotic changes in the intestinal mucosa: intracellular vacuolization of epithelial cells, desquamation and necrosis of the villous epithelium, intercellular edema, and infiltration of lymphocytes and histiocytes. The detectable changes in the morphology of the intestinal mucosa preceded the increase in fluid accumulation in intestinal loops. Intraluminal injection of botulinum C2 toxin also induced the leakage of plasma protein into the intestinal lumen as determined by the extravasation of Evans blue. In contrast to botulinum C2 toxin, cholera and Clostridium perfringens enterotoxin controls caused a very slight protein leakage, although these toxins induced marked fluid accumulation in intestinal loops. The results indicate that the mode of action of botulinum C2 toxin in eliciting the secretory response is distinguishable from those of cholera and C. perfringens enterotoxins and suggest that botulinum C2 toxin induces the secretory response by cytopathic effect(s) on the epithelial cells of the intestine.