DNA-based and alphavirus-vectored immunisation with prM and E proteins elicits long-lived and protective immunity against the flavivirus, Murray Valley encephalitis virus
DNA-based and alphavirus-vectored immunisation with prM and E proteins elicits long-lived and protective immunity against the flavivirus, Murray Valley encephalitis virus
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DOI：
10.1006/viro.1998.9357
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发表时间：
1998-10-10
期刊：
影响因子：
3.7
通讯作者：
Lobigs, M
中科院分区：
文献类型：
--
作者：
Colombage, G;Hall, R;Lobigs, M
用编码黄病毒墨利谷脑炎病毒（MVE）膜蛋白prM和E的质粒进行免疫原性和免疫保护效果的研究。基因枪介导的编码prM和E蛋白的DNA皮内递送在三种近交小鼠品系中引发了长期的病毒中和抗体反应，并提供了高滴度MVE接种的保护。肌内注射DNA疫苗也能诱导mve特异性抗体，使其抵抗活病毒的攻击，但在体外不能降低病毒的传染性。用prM和E编码的质粒接种两种基于dna的疫苗可产生具有不同IgG亚型的体液免疫。通过基因枪皮内DNA疫苗接种后，mve特异性IgG(1)抗体普遍存在，而通过肌肉内途径DNA免疫或感染活病毒时则未检测到。我们还测试了塞姆利基森林病毒复制子作为黄病毒prM和E蛋白亚单位疫苗的载体。在接种包装重组复制子颗粒的小鼠中，单周期感染引发了持久的、mve特异性的和病毒中和的抗体反应。(C) 1998学术出版社。
The immunogenicity and protective efficacy of DNA-based vaccination with plasmids encoding the membrane proteins prM and E of the flavivirus Murray Valley encephalitis virus (MVE) were investigated. Gene gun-mediated intradermal delivery of DNA encoding the prM and E proteins elicited long-lived, virus-neutralising antibody responses in three inbred strains of mice and provided protection from challenge with a high titer inoculum of MVE. Intramuscular DNA vaccination by needle injection also induced MVE-specific antibodies that conferred resistance to challenge with live Virus but failed to reduce virus infectivity in vitro. The two routes of DNA-based vaccination with prM and E encoding plasmids resulted in humoral immunty with distinct IgG subtypes. MVE-specific IgG(1) antibodies were always prevalent after intradermal DNA Vaccination via a gene gun but not detected when mice were immunised with DNA by the intramuscular route or infected with live virus. We also tested a Semliki Forest virus replicon as vector for a flavivirus prM and E protein-based subunit vaccine. Single-cycle infections in mice vaccinated with packaged recombinant replicon particles elicited durable, MvE-specific, and virus-neutralising antibody responses. (C) 1998 Academic Press.