Determining the topology of stable protein-DNA complexes
Determining the topology of stable protein-DNA complexes
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DOI：
10.1042/bst20130004
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发表时间：
2013-04-01
影响因子：
3.9
通讯作者：
Vazquez, Mariel
中科院分区：
文献类型：
--
作者：
Darcy, Isabel K.;Vazquez, Mariel
差异拓扑是一种实验技术，可用于揭示稳定的蛋白质-DNA复合物中两个或多个DNA片段所采用的拓扑结构。不同的拓扑结构也被用来检测反应途径中的中间体，并研究DNA超螺旋的作用。在这篇文章中，我们回顾了应用于Mu转座体的差分拓扑结构。所讨论的工具可以应用于任何稳定的核蛋白复合物。
Difference topology is an experimental technique that can be used to unveil the topological structure adopted by two or more DNA segments in a stable protein-DNA complex. Difference topology has also been used to detect intermediates in a reaction pathway and to investigate the role of DNA supercoiling. In the present article, we review difference topology as applied to the Mu transpososome. The tools discussed can be applied to any stable nucleoprotein complex.