TOWARD RULES RELATING ZINC FINGER PROTEIN SEQUENCES AND DNA-BINDING SITE PREFERENCES
TOWARD RULES RELATING ZINC FINGER PROTEIN SEQUENCES AND DNA-BINDING SITE PREFERENCES
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DOI：
10.1073/pnas.89.16.7345
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发表时间：
1992-08-15
影响因子：
11.1
通讯作者：
BERG, JM
中科院分区：
文献类型：
--
作者：
DESJARLAIS, JR;BERG, JM
Cys2-His2型锌指蛋白由串联结构域阵列组成，其中每个结构域似乎通过三个关键残基与三个相邻的DNA碱基对接触。我们利用大量锌指蛋白序列数据库的分析信息，在Sp1的DNA结合域内设计并制备了一系列中央锌指蛋白的变体。通过在三个接触位置（下划线）中的两个位置进行系统变化，实现了对形式为5‘-GGGGN(G或T)GGG-3’的序列的相对特异性识别。这些结果为可能发展成代码的规则提供了基础，该代码将允许设计具有预选DNA位点特异性的锌林格蛋白。
Zinc finger proteins of the Cys2-His2 type consist of tandem arrays of domains, where each domain appears to contact three adjacent base pairs of DNA through three key residues. We have designed and prepared a series of variants of the central zinc ringer within the DNA binding domain of Sp1 by using information from an analysis of a large data base of zinc finger protein sequences. Through systematic variations at two of the three contact positions (underlined), relatively specific recognition of sequences of the form 5'-GGGGN(G or T)GGG-3' has been achieved. These results provide the basis for rules that may develop into a code that will allow the design of zinc ringer proteins with preselected DNA site specificity.