Pleiotropic upregulation of Na(+)-dependent cotransporters by retinoic acid in opossum kidney cells.
Pleiotropic upregulation of Na(+)-dependent cotransporters by retinoic acid in opossum kidney cells.
复制标题
负鼠肾细胞中视黄酸对Na(+)依赖性协同转运蛋白的多效性上调。
DOI：
10.1152/ajprenal.1997.273.3.f438
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Dousa,TP
中科院分区：
文献类型：
--
作者：
deToledo,FG;Beers,KW;Dousa,TP
全反式视黄酸（atRA）是细胞生长和分化的调节剂。我们研究了atRA是否可以上调负鼠肾（OK）细胞中的Na（+）依赖性协同转运蛋白，从而增加个体发育早期阶段生长所需的几种溶质从小管液中的摄取。在OK细胞中，与atRA孵育24小时可增加磷酸盐、L-脯氨酸、L-谷氨酸和SO（4）2-的Na+梯度依赖性共转运，其增加程度与放线菌素D预处理所阻止的程度相似（约40%）。相反，其他Na（+）依赖性转运蛋白，Na（+）-K（+）-腺苷三磷酸酶，γ-谷氨酰转肽酶和亮氨酸氨肽酶的活性，则未受到atRA的影响。[3 H]胸苷掺入测定的细胞增殖没有增加atRA。atRA和磷酸盐剥夺对Na（+）-Pi共转运的刺激作用表现为加和性，而atRA和3，5，3 '-三碘甲状腺原氨酸的联合作用则没有。atRA刺激LLC-PK 1细胞中Na（+）-Pi共转运的时间过程和程度与在OK细胞中观察到的相似。我们的结论是，atRA刺激几个Na（+）-依赖的协同转运蛋白通过基因组机制，并可能代表一个同步适应的营养需求的早期阶段的个体发育。
All-trans-retinoic acid (atRA) is a regulator of cellular growth and differentiation. We investigated whether atRA can upregulate Na(+)-dependent cotransporters in opossum kidney (OK) cells and thus increase uptake from tubular fluid of several solutes needed for growth during early stages of ontogenesis. In OK cells, incubation with atRA for 24 h increased the Na+ gradient-dependent cotransports of phosphate, L-proline, L-glutamic acid, and SO(4)2- by a similar degree (approximately 40%) that was prevented by pretreatment with actinomycin D. In contrast, activities of other Na(+)-dependent transporters, Na(+)-K(+)-adenosinetriphosphatase, gamma-glutamyltranspeptidase, and leucine aminopeptidase, were unchanged by atRA. Cell proliferation determined by [3H]thymidine incorporation was not increased by atRA. The stimulatory effects of atRA and phosphate deprivation on Na(+)-Pi cotransport demonstrated additivity, whereas the combination of atRA and 3,5,3'-triiodothyronine did not. atRA stimulated Na(+)-Pi cotransport in LLC-PK1 cells with an analogous time course and to a similar extent as observed in OK cells. We conclude that atRA stimulates several Na(+)-dependent cotransporters via a genomic mechanism and may represent a synchronous adaptation to nutritional requirements of early phases of ontogenesis.