Dynamics of enhancer-promoter communication during differentiation-induced gene activation
Dynamics of enhancer-promoter communication during differentiation-induced gene activation
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DOI：
10.1016/s1097-2765(02)00786-4
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发表时间：
2002-12-01
期刊：
影响因子：
16
通讯作者：
Talianidis, I
中科院分区：
文献类型：
--
作者：
Hatzis, P;Talianidis, I
我们分析了在肠上皮细胞分化过程中，HNF-4 α基因初始激活后，HNF-4 α调控区因子的募集顺序。在近端启动子和上游增强子区域的调节复合物的最初独立组装之后，跟踪在增强子上形成的整个DNA-蛋白质复合物沿着插入DNA，直到它遇到近端启动子。这种运动与组蛋白超乙酰化的单向扩散相关。转录起始与稳定的增强子-启动子复合物的形成和位于转录起始位点的核小体的重塑相一致。结果提供了实验证据，参与一个动态的过程最终在增强子启动子通信过程中的长距离基因激活。
We analyzed the order of recruitment of factors to the HNF-4alpha regulatory regions upon the initial activation of the gene during enterocyte differentiation. An initially independent assembly of regulatory complexes at the proximal promoter and the upstream enhancer regions was followed by the tracking of the entire DNA-protein complex formed on the enhancer along the intervening DNA until it encountered the proximal promoter. This movement correlated with a unidirectional spreading of histone hyperacetylation. Transcription initiation coincided with the formation of a stable enhancer-promoter complex and remodeling of the nucleosome situated at the transcription start site. The results provide experimental evidence for the involvement of a dynamic process culminating in enhancer-promoter communication during long-distance gene activation.