The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein
The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein
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DOI：
10.1093/emboj/17.20.6061
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发表时间：
1998-10-15
期刊：
影响因子：
11.4
通讯作者：
Storz, G
中科院分区：
文献类型：
--
作者：
Zhang, AX;Altuvia, S;Storz, G
OxyS调节RNA整合了对过氧化氢的适应性反应和其他细胞应激反应，并保护DNA免受损伤。OxyS靶点中包括RNA聚合酶的rpoS编码的sigma（S）亚基。sigma（S）是由渗透胁迫、饥饿和进入稳定期诱导的基因的中心调节因子。我们研究了OxyS抑制rpoS表达的机制，发现OxyS RNA抑制rpoS信息的翻译。这种抑制依赖于hfq编码的RNA结合蛋白（也称为宿主因子I，HF-I）。免疫共沉淀和凝胶迁移率变化实验显示，OxyS RNA结合Hfq，表明OxyS通过改变Hfq活性抑制rpoS翻译。
The OxyS regulatory RNA integrates the adaptive response to hydrogen peroxide with other cellular stress responses and protects against DNA damage. Among the OxyS targets is the rpoS-encoded sigma(S) subunit of RNA polymerase. sigma(S) is a central regulator of genes induced by osmotic stress, starvation and entry into stationary phase. We examined the mechanism whereby OxyS represses rpoS expression and found that the OxyS RNA inhibits translation of the rpoS message. This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I). Co-immunoprecipitation and gel mobility shift experiments revealed that the OxyS RNA binds Hfq, suggesting that OxyS represses rpoS translation by altering Hfq activity.